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BIOL388/S19:Class Journal Week 11: Difference between revisions
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#Discuss amongst yourselves whether or not the Tai et al (2007) journal club paper gives you enough information to perform such a simulation. | #Discuss amongst yourselves whether or not the Tai et al (2007) journal club paper gives you enough information to perform such a simulation. | ||
#What parameters are explicitly given? | #What parameters are explicitly given? | ||
The researchers only explicitly provide the dilution rate and the volume in the container. Searching back through their previous paper is necessary to find nutrient concentrations. The parameters that were explicitly stated are listed below: | |||
* dilution rate = 0.03 h^-1 | |||
* volume in container = 1 L | |||
* provide nutrient concentrations by referencing methods from previous study (Tai et al., 2005) | |||
** glucose limited culture | |||
*** 5.0 g liter^1 (NH4)2SO4 | |||
*** 3.0 g liter^1 KH2PO4 | |||
*** 0.5 g liter^1 MgSO47H2O | |||
*** 25 g liter^1 glucose | |||
** ammonia limited culture | |||
*** 0.65 g liter^1(NH4)2SO4 | |||
*** 5.75 g liter^1 K2SO4 | |||
*** 3.0 g liter^1 KH2PO4 | |||
*** 0.5 g liter^1 MgSO47H2O | |||
*** 46 g liter^1 glucose | |||
#Can you back any others out from the data tables? | #Can you back any others out from the data tables? | ||
#To what in the model do the quantities in Table 1 correspond? | #To what in the model do the quantities in Table 1 correspond? | ||
A temperature probe connected to a cryostat controlled cultures grown at 12°C. | |||
Cultures were grown in a defined synthetic medium that was limited by | |||
carbon or by nitrogen with all other growth requirements in excess as previously described (Tai et al., 2005). The dilution rate was set at 0.03 h1 with pH | |||
measured on-line and kept constant at 5.0 by automatic addition of 2 M KOH | |||
using an Applikon ADI 1030 Biocontroller. The stirrer speed was set to 600 | |||
rpm. Anaerobic growth and steady-state conditions were maintained as previously described (Tai et al., 2005). Biomass dry weight, metabolites, dissolved | |||
oxygen, and gas profiles were constant for at least three volume changes | |||
before sampling. | |||
==[[User:EdwardRyanTalatala|Edward's Journal Reflection]]== | ==[[User:EdwardRyanTalatala|Edward's Journal Reflection]]== | ||
