96
edits
BME100 s2018:Group9 W0800 L4: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Research and Development
| Line 115: | Line 115: | ||
'''What happens to the components during each step of thermal cycling?''' | '''What happens to the components during each step of thermal cycling?''' | ||
Thermal cycling consists of steps that make DNA replicate into billions of copies. The intial step is where the DNA is heated up and causes the DNA strands to split; also providing access to the primers. During the second step (denature)an excess amount of primers are added. The reaction mixture must be cooled that way double strands of DNA are able to form again and they will always bind to primers. During the third step (Anneal), a mixture containing deocyribosnucleotides, will be combined with an enzyme called Taq polymerase. The enzyme matches up the nucleotides during this step, in order to make the DNA sequence. The Final step to the polymerase reaction is simply replicating the exact same steps above multiple times in order to make billions of copies of the region of the DNA template. | |||
'''Base-pairing. driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each listed? Adenine: Thymine: Cytosine: Guanine: ''' | '''Base-pairing. driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each listed? Adenine: Thymine: Cytosine: Guanine: ''' | ||
