96
edits
BME100 s2018:Group9 W0800 L4: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Protocol
| Line 72: | Line 72: | ||
'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
<!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | <!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | ||
* Step 1: Extract DNA from cells. | |||
* step 2: Place the DNA into a special PCR tube. | |||
* Step 3: Add primer 1 to the PCR tube. | |||
* Step 4: Add primer 2(will attach to the second site) | |||
* Step 5: Add nucleotide to the PCR tube. | |||
* Step 6: Add DNA Polymerase to the PCR tube. | |||
* Step 7: Place PCR tube, with all components, into a DNA thermal cycler. | |||
'''OpenPCR program''' | '''OpenPCR program''' | ||
