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BME100 s2018:Group9 W0800 L4: Difference between revisions
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'''Materials''' | '''Materials''' | ||
<!-- Record all of the materials you will need for PCR --> | <!-- Record all of the materials you will need for PCR --> | ||
* Lab coat and disposable gloves | |||
* PCR reacon | |||
mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl | mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl | ||
2, and dNTP’s | 2, and dNTP’s | ||
(hp://www.promega.com/resources/protocols/product‐informaon‐sheets/g/gotaq‐colorless | (hp://www.promega.com/resources/protocols/product‐informaon‐sheets/g/gotaq‐colorless | ||
‐master‐mix‐m714‐protocol/) | ‐master‐mix‐m714‐protocol/) | ||
* DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes | |||
have the same forward primer and reverse primer | have the same forward primer and reverse primer | ||
* A strip of empty PCR tubes | |||
* Disposable pipee | |||
ps: | ps: | ||
only use each only once. Never reuse disposable pipee | only use each only once. Never reuse disposable pipee | ||
| Line 43: | Line 43: | ||
If you do, | If you do, | ||
the samples will become cross‐contaminated | the samples will become cross‐contaminated | ||
* Cup for discarded ps | |||
* Micropipeor | |||
* OpenPCR machine: s | |||
