BME100 s2018:Group9 W0800 L4

BME 100 Spring 2018	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Anna Avila Name: Taylor Dinubilo Name: Tyler Thompson Name: Zoey Wirtes Name: student Role(s) Name: student Role(s) LAB 4 WRITE-UP Protocol Materials Lab coat and disposable gloves PCR reacon mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl 2, and dNTP’s (hp://www.promega.com/resources/protocols/product‐informaon‐sheets/g/gotaq‐colorless ‐master‐mix‐m714‐protocol/) DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer A strip of empty PCR tubes Disposable pipee ps: only use each only once. Never reuse disposable pipee ps. If you do, the samples will become cross‐contaminated Cup for discarded ps Micropipeor OpenPCR machine: s PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G# + Positive control none G# - Negative control none G# 1-1 Patient 1, replicate 1 G# 1-2 Patient 1, replicate 2 G# 1-3 Patient 1, replicate 3 G# 2-1 Patient 2, replicate 1 G# 2-2 Patient 2, replicate 2 G# 2-3 Patient 2, replicate 3 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... OpenPCR program Research and Development PCR - The Underlying Technology SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing