User contributions for Anjali Bisaria

31 March 2012

• 13:3313:33, 31 March 2012 diff hist +5,436‎ N McClean:LFM Proline ‎ New page: ==Overview== Standard LFM Media but with Proline as the nitrogen source. As always, leave out glucose if the carbon source is variable and use amino acid mix as needed. ==Final Desired... current

30 September 2011

• 08:4308:43, 30 September 2011 diff hist +174‎ McClean:Links ‎No edit summary

14 August 2011

• 21:3821:38, 14 August 2011 diff hist 0‎ McClean:Protocols ‎ →‎Yeast
• 21:3821:38, 14 August 2011 diff hist +56‎ McClean:Protocols ‎ →‎Yeast
• 21:3821:38, 14 August 2011 diff hist +1,239‎ N McClean:Mating Type Testers ‎ New page: ==Overview== How to make mating type tester plates from yMM421 and yMM422 and their drug resistant derivatives. ==Materials== yMM421/422 colonies growing on YPD YPD and/or Drug plates dH<... current
• 21:2821:28, 14 August 2011 diff hist +1,615‎ McClean:Basic Chemostat Guide ‎No edit summary current
• 21:1221:12, 14 August 2011 diff hist +1,624‎ McClean:Basic Chemostat Guide ‎No edit summary
• 20:0520:05, 14 August 2011 diff hist +2,415‎ McClean:Chemostat Metabolic Cycle ‎No edit summary current
• 09:4209:42, 14 August 2011 diff hist +69‎ McClean:Protocols ‎ →‎Yeast
• 09:4109:41, 14 August 2011 diff hist +1,150‎ McClean:Chemostat Metabolic Cycle ‎No edit summary
• 09:3009:30, 14 August 2011 diff hist +282‎ N McClean:Chemostat Metabolic Cycle ‎ New page: ==Overview== This is a guide on how to induce the Metabolic Cccle in FY diploid Yeast Strains (First used on 8/5/11-8/14/11) and subsequently sample the culture for various things. To see...
• 09:2709:27, 14 August 2011 diff hist +98‎ McClean:Basic Chemostat Guide ‎No edit summary
• 09:2709:27, 14 August 2011 diff hist +60‎ McClean:Protocols ‎ →‎Yeast
• 09:2509:25, 14 August 2011 diff hist +435‎ McClean:Basic Chemostat Guide ‎No edit summary
• 09:1809:18, 14 August 2011 diff hist +63‎ McClean:Protocols ‎No edit summary
• 09:1709:17, 14 August 2011 diff hist +35‎ N 96-well plate.pdf ‎ 96-well plate.pdf moved to McClean:96-well plate: Not put in right index current
• 09:1709:17, 14 August 2011 diff hist 0‎ m McClean:96-well plate ‎ 96-well plate.pdf moved to McClean:96-well plate: Not put in right index current
• 09:1609:16, 14 August 2011 diff hist +214‎ N McClean:96-well plate ‎ New page: Use this sheet in Lab Notebooks to annotate reaction conditions, strains, or glycerol stock identities, etc. Contains 2 8 (A-H) x 12 (1-12) grids that can be printed out and taped in. [[...
• 09:1009:10, 14 August 2011 diff hist +75‎ N File:96-well plate.pdf ‎ A PDF that has a grid of a 96 well plate to print and use in lab notebooks. current

13 August 2011

• 16:2316:23, 13 August 2011 diff hist +318‎ McClean:FISH ‎ →‎Protocol current

10 August 2011

• 09:4309:43, 10 August 2011 diff hist +885‎ McClean:Basic Chemostat Guide ‎ →‎Vessels
• 09:3609:36, 10 August 2011 diff hist +1,604‎ McClean:Basic Chemostat Guide ‎No edit summary
• 09:2309:23, 10 August 2011 diff hist +351‎ N McClean:Basic Chemostat Guide ‎ New page: ==Overview== This is a simple introduction into how to set up a chemostat experiment using the Botstein Lab Chemostat Room. Adapted from the see [http://www.princeton.edu/genomics/botstei...

8 August 2011

• 16:4016:40, 8 August 2011 diff hist +3‎ McClean ‎No edit summary
• 16:3716:37, 8 August 2011 diff hist +33‎ McClean:Protocols ‎ →‎PCR
• 16:3616:36, 8 August 2011 diff hist +702‎ N McClean:dNTPs ‎ New page: We use Roche Diagnostics dNTP set. Each individual dNTP comes as 10 μmol in 100 mM solution for a total of 4 tubes. Each lids labeled with the nucleic acid it contains. The kits are ...

27 July 2011

• 10:5110:51, 27 July 2011 diff hist +61‎ McClean:Protocols ‎No edit summary
• 10:5010:50, 27 July 2011 diff hist +1‎ McClean:LFA Membranes ‎No edit summary
• 10:5010:50, 27 July 2011 diff hist −239‎ McClean:LFA Membranes ‎No edit summary
• 10:4910:49, 27 July 2011 diff hist +874‎ McClean:LFA Membranes ‎ →‎Final Composition of Media (100 mL total)
• 10:4210:42, 27 July 2011 diff hist +1,387‎ N McClean:LFA Membranes ‎ New page: ==Overview== Use this protocol to make small Low Fluorescence Agar Membranes. Adapted from Wong et al (2010) for use with the FCS2. We adapted the original protocol for bacterial membran...
• 10:3410:34, 27 July 2011 diff hist +49‎ McClean:Protocols ‎No edit summary

26 July 2011

• 08:5508:55, 26 July 2011 diff hist +960‎ N McClean:Scope Settings ‎ New page: ==Filter Settings== There are now and additional 2 sets of filters that have been installed for FRET. To use FRET imaging there are YFP and CFP Excitation and EMission Filters that must be... current

24 July 2011

• 10:1010:10, 24 July 2011 diff hist +1,072‎ N McClean: Dilution of Oligos ‎ New page: Dilution of Oligos *We order most oligos from IDT[http://www.idtdna.com/Home/Home.aspx|IDT]. *When you receive the oligos, the IDT Spec Sheet will tell you the amount of oligo in nMoles *L...
• 10:0610:06, 24 July 2011 diff hist −1‎ McClean:Protocols ‎No edit summary
• 10:0510:05, 24 July 2011 diff hist +52‎ McClean:Protocols ‎No edit summary
• 10:0410:04, 24 July 2011 diff hist +1,562‎ N McClean:Touchdown PCR ‎ New page: ==Background== Use this PCR to avoid primers amplifying nonspecific sequences. The procedure is based off of 2 principles. First, the specificity of the products is based off the annealing... current

8 July 2011

• 09:2709:27, 8 July 2011 diff hist +62‎ McClean:Protocols ‎No edit summary
• 09:2709:27, 8 July 2011 diff hist +3,049‎ N McClean:Random Spore Prep ‎ New page: Category:Template <!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! --> ==Overview== A way to enrich for haploid cells from a mix of haploids and diploids. This is an alte...
• 09:0309:03, 8 July 2011 diff hist −9‎ McClean:Research ‎No edit summary current
• 09:0209:02, 8 July 2011 diff hist +4,915‎ N McClean:Research ‎ New page: Projects ==Signal Processing by the Yeast Hyperosmotic Response Pathway== How quickly and accurately information propagates through a signaling pathway determines how it functions within ...

30 June 2011

• 10:2510:25, 30 June 2011 diff hist +59‎ McClean:Protocols ‎No edit summary
• 10:2210:22, 30 June 2011 diff hist +8‎ McClean:Protocols ‎No edit summary
• 10:2110:21, 30 June 2011 diff hist +23‎ N McClean:Protocols ‎ New page: Back to McClean Lab
• 10:2010:20, 30 June 2011 diff hist +9‎ McClean ‎No edit summary
• 10:2010:20, 30 June 2011 diff hist +8‎ McClean ‎No edit summary

28 June 2011

• 13:4313:43, 28 June 2011 diff hist +2,234‎ McClean:LFM Recipe ‎No edit summary
• 13:0713:07, 28 June 2011 diff hist +3,289‎ N McClean:LFM Recipe ‎ New page: Standard media except with low-fluorescence yeast nitrogen base. (Yeast nitrogen base without riboflavin and folic acid.) Final Desired Composition of Yeast Nitrogen Base (Note: Ca¬2Cl...
• 11:5511:55, 28 June 2011 diff hist −470‎ User:Anjali Bisaria/Notebook/YMC ‎ →‎Project Description/Abstract
• 11:5411:54, 28 June 2011 diff hist +122‎ User:Anjali Bisaria/Notebook/YMC/2011/04/19 ‎ →‎Entry title