User contributions for Anjali Bisaria

20:3320:33, 31 March 2012 diff hist +5,436 N McClean:LFM Proline New page: ==Overview== Standard LFM Media but with Proline as the nitrogen source. As always, leave out glucose if the carbon source is variable and use amino acid mix as needed. ==Final Desired... current

04:3804:38, 15 August 2011 diff hist 0 McClean:Protocols →Yeast
04:3804:38, 15 August 2011 diff hist +56 McClean:Protocols →Yeast
04:3804:38, 15 August 2011 diff hist +1,239 N McClean:Mating Type Testers New page: ==Overview== How to make mating type tester plates from yMM421 and yMM422 and their drug resistant derivatives. ==Materials== yMM421/422 colonies growing on YPD YPD and/or Drug plates dH<... current
04:2804:28, 15 August 2011 diff hist +1,615 McClean:Basic Chemostat Guide No edit summary current
04:1204:12, 15 August 2011 diff hist +1,624 McClean:Basic Chemostat Guide No edit summary
03:0503:05, 15 August 2011 diff hist +2,415 McClean:Chemostat Metabolic Cycle No edit summary current

16:4216:42, 14 August 2011 diff hist +69 McClean:Protocols →Yeast
16:4116:41, 14 August 2011 diff hist +1,150 McClean:Chemostat Metabolic Cycle No edit summary
16:3016:30, 14 August 2011 diff hist +282 N McClean:Chemostat Metabolic Cycle New page: ==Overview== This is a guide on how to induce the Metabolic Cccle in FY diploid Yeast Strains (First used on 8/5/11-8/14/11) and subsequently sample the culture for various things. To see...
16:2716:27, 14 August 2011 diff hist +98 McClean:Basic Chemostat Guide No edit summary
16:2716:27, 14 August 2011 diff hist +60 McClean:Protocols →Yeast
16:2516:25, 14 August 2011 diff hist +435 McClean:Basic Chemostat Guide No edit summary
16:1816:18, 14 August 2011 diff hist +63 McClean:Protocols No edit summary
16:1716:17, 14 August 2011 diff hist +35 N 96-well plate.pdf 96-well plate.pdf moved to McClean:96-well plate: Not put in right index current
16:1716:17, 14 August 2011 diff hist 0 m McClean:96-well plate 96-well plate.pdf moved to McClean:96-well plate: Not put in right index current
16:1616:16, 14 August 2011 diff hist +214 N McClean:96-well plate New page: Use this sheet in Lab Notebooks to annotate reaction conditions, strains, or glycerol stock identities, etc. Contains 2 8 (A-H) x 12 (1-12) grids that can be printed out and taped in. [[...
16:1016:10, 14 August 2011 diff hist +75 N File:96-well plate.pdf A PDF that has a grid of a 96 well plate to print and use in lab notebooks. current

16:4316:43, 10 August 2011 diff hist +885 McClean:Basic Chemostat Guide →Vessels
16:3616:36, 10 August 2011 diff hist +1,604 McClean:Basic Chemostat Guide No edit summary
16:2316:23, 10 August 2011 diff hist +351 N McClean:Basic Chemostat Guide New page: ==Overview== This is a simple introduction into how to set up a chemostat experiment using the Botstein Lab Chemostat Room. Adapted from the see [http://www.princeton.edu/genomics/botstei...

23:4023:40, 8 August 2011 diff hist +3 McClean No edit summary
23:3723:37, 8 August 2011 diff hist +33 McClean:Protocols →PCR
23:3623:36, 8 August 2011 diff hist +702 N McClean:dNTPs New page: We use Roche Diagnostics dNTP set. Each individual dNTP comes as 10 μmol in 100 mM solution for a total of 4 tubes. Each lids labeled with the nucleic acid it contains. The kits are ...

17:5117:51, 27 July 2011 diff hist +61 McClean:Protocols No edit summary
17:5017:50, 27 July 2011 diff hist +1 McClean:LFA Membranes No edit summary
17:5017:50, 27 July 2011 diff hist −239 McClean:LFA Membranes No edit summary
17:4917:49, 27 July 2011 diff hist +874 McClean:LFA Membranes →Final Composition of Media (100 mL total)
17:4217:42, 27 July 2011 diff hist +1,387 N McClean:LFA Membranes New page: ==Overview== Use this protocol to make small Low Fluorescence Agar Membranes. Adapted from Wong et al (2010) for use with the FCS2. We adapted the original protocol for bacterial membran...
17:3417:34, 27 July 2011 diff hist +49 McClean:Protocols No edit summary

15:5515:55, 26 July 2011 diff hist +960 N McClean:Scope Settings New page: ==Filter Settings== There are now and additional 2 sets of filters that have been installed for FRET. To use FRET imaging there are YFP and CFP Excitation and EMission Filters that must be... current

17:1017:10, 24 July 2011 diff hist +1,072 N McClean: Dilution of Oligos New page: Dilution of Oligos *We order most oligos from IDT[http://www.idtdna.com/Home/Home.aspx|IDT]. *When you receive the oligos, the IDT Spec Sheet will tell you the amount of oligo in nMoles *L...
17:0617:06, 24 July 2011 diff hist −1 McClean:Protocols No edit summary
17:0517:05, 24 July 2011 diff hist +52 McClean:Protocols No edit summary
17:0417:04, 24 July 2011 diff hist +1,562 N McClean:Touchdown PCR New page: ==Background== Use this PCR to avoid primers amplifying nonspecific sequences. The procedure is based off of 2 principles. First, the specificity of the products is based off the annealing... current

16:2716:27, 8 July 2011 diff hist +62 McClean:Protocols No edit summary
16:2716:27, 8 July 2011 diff hist +3,049 N McClean:Random Spore Prep New page: Category:Template  ==Overview== A way to enrich for haploid cells from a mix of haploids and diploids. This is an alte...
16:0316:03, 8 July 2011 diff hist −9 McClean:Research No edit summary current
16:0216:02, 8 July 2011 diff hist +4,915 N McClean:Research New page: Projects ==Signal Processing by the Yeast Hyperosmotic Response Pathway== How quickly and accurately information propagates through a signaling pathway determines how it functions within ...

20:4320:43, 28 June 2011 diff hist +2,234 McClean:LFM Recipe No edit summary
20:0720:07, 28 June 2011 diff hist +3,289 N McClean:LFM Recipe New page: Standard media except with low-fluorescence yeast nitrogen base. (Yeast nitrogen base without riboflavin and folic acid.) Final Desired Composition of Yeast Nitrogen Base (Note: Ca¬2Cl...
18:5518:55, 28 June 2011 diff hist −470 User:Anjali Bisaria/Notebook/YMC →Project Description/Abstract
18:5418:54, 28 June 2011 diff hist +122 User:Anjali Bisaria/Notebook/YMC/2011/04/19 →Entry title

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