Schumer lab: DNA extraction
From OpenWetWareJump to navigationJump to search
- The low coverage whole genome sequencing we do in the lab requires 5ng of DNA per sample that should be 10kb or above.
- We use the Agencourt DNAdvance kit (Beckman Coulter A48705) kit according to manufacturer suggestions except in half reactions.
- See DNA_extraction_using_AgencourtDNAdvance_kit_halfrxns_smb.docx in the Protocols folder in Box or email email@example.com for the protocol and all necessary reagents and consumables.
- Our standard format can be found in /Box/Schumer_lab_resources/Extractions_and_library_preps/Extractions