Schumer lab: DNA extraction

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  • The low coverage whole genome sequencing we do in the lab requires 5ng of DNA per sample that should be 10kb or above.
  • We use the Agencourt DNAdvance kit (Beckman Coulter A48705) kit according to manufacturer suggestions except in half reactions.
  • See DNA_extraction_using_AgencourtDNAdvance_kit_halfrxns_smb.docx in the Protocols folder in Box or email smbanerj@stanford.edu for the protocol and all necessary reagents and consumables.
  • Our standard format can be found in /Box/Schumer_lab_resources/Extractions_and_library_preps/Extractions