SBB10 protocols

All these protocols in one big list

Polymerase Reactions

Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
QuikChange

Cleanup Reactions

Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions

EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations

Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA

Assays

Running an Analytical Gel

Other

LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation
ELISA