Prince:Mass Spectrometry

Home        Lab Members         Research         Publications         Internal         Mass Spec         Contact        

New Users

We recommend you peruse this site and become more familiar with the content. Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you.

Introduction to Mass Spectrometry

Sample Preparation

Proven Methods:

• FASP (filter aided sample preparation)
• In Gel FASP
• Yeast FASP

Side Notes:

• Reduction & Alkylation
• Microwave Digestion

Wet Lab Rules

• Lab Practices

Equipment

• Tech Support
• MS Prep Consumables
• MS Consumables
• Miscellaneous Tips

Gases

[From the wall out]

• Helium UHP [LTQ] (40psi)
• Nitrogen UHP [HCD] (100psi)
• Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi)
• Nitrogen Normal (100psi) [for UPLC]

UPLC

• Troubleshooting the Eksigent UPLC system
• UPLC (Fittings/tubing/flow rates)
  • 10 micron needles provide best nano-spray at 200-300 nl/min

Tuning/Calibration

• Tuning and Calibration
• Klaus's Cleaning Mix

Mascot Restart

 user@jp1:~$ ls /etc/init.d/mascot
/etc/init.d/mascot
user@jp1:~$ sudo /etc/init.d/mascot status
[sudo] password for user:
user@jp1:~$ sudo /etc/init.d/mascot restart
 * Mascot monitor is not running
 * Mascot monitor started
user@jp1:~$

You may also try this.

If the mascot daemon is not running:

[You need to have sudo priviledges on jp1]

In the command line enter;

sudo /mascot/bin/ms-monitor.exe

From home, this could be done, after vpn-ing into the chemistry network with a single ssh command:

ssh -t <USERNAME>@jp1.chem.byu.edu 'sudo /mascot/bin/ms-monitor.exe'

Scheduling

You may access the scheduling site here:

Orbitrap Scheduling