Prince:Microwave Digestion

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  1. Sun W, Gao S, Wang L, Chen Y, Wu S, Wang X, Zheng D, and Gao Y. Microwave-assisted protein preparation and enzymatic digestion in proteomics. Mol Cell Proteomics. 2006 Apr;5(4):769-76. DOI:10.1074/mcp.T500022-MCP200 | PubMed ID:16339992 | HubMed [P1]
  2. Reddy PM, Hsu WY, Hu JF, and Ho YP. Digestion completeness of microwave-assisted and conventional trypsin-catalyzed reactions. J Am Soc Mass Spectrom. 2010 Mar;21(3):421-4. DOI:10.1016/j.jasms.2009.11.006 | PubMed ID:20044269 | HubMed [P2]
  3. Hahn HW, Rainer M, Ringer T, Huck CW, and Bonn GK. Ultrafast microwave-assisted in-tip digestion of proteins. J Proteome Res. 2009 Sep;8(9):4225-30. DOI:10.1021/pr900188x | PubMed ID:19639939 | HubMed [P3]
  4. Hasan N, Wu HF, Li YH, and Nawaz M. Two-step on-particle ionization/enrichment via a washing- and separation-free approach: multifunctional TiO2 nanoparticles as desalting, accelerating, and affinity probes for microwave-assisted tryptic digestion of phosphoproteins in ESI-MS and MALDI-MS: comparison with microscale TiO2. Anal Bioanal Chem. 2010 Apr;396(8):2909-19. DOI:10.1007/s00216-010-3573-3 | PubMed ID:20232060 | HubMed [P4]

    Note that this works in conjunction with phosphoproteomic analysis.

  5. Lin S, Yao G, Qi D, Li Y, Deng C, Yang P, and Zhang X. Fast and efficient proteolysis by microwave-assisted protein digestion using trypsin-immobilized magnetic silica microspheres. Anal Chem. 2008 May 15;80(10):3655-65. DOI:10.1021/ac800023r | PubMed ID:18407620 | HubMed [P5]

All Medline abstracts: PubMed | HubMed