Kubke Lab:Research/CND/Records2010-2011Summer
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Experimental records for the Cranial Nerve Development Project- 2010-2011 Summer research
This page lists and links to the detailed experimental records for Summer Research 2010-2011
Embryo name | Stage | Date | Procedure | Nerve | Comments |
---|---|---|---|---|---|
MH001 | ST28 | 25/11/2010 | Cryostat Tutorial | N/A | Stage confirmed by Fabiana |
MH002 | ST28 | 3/12/2010 | Assessing cryostat parameters for ST28 | N/A | H&E stained slides.Poor quality |
MH003 | ST20-22 | 13/12/2010 | Fabiana's cryostat session | N/A | N/A |
MH004 | ST28-29 | 16/12/2010 | Fabiana's cryostat session | N/A | feedback from results found here |
MH005 | ST28-29 | 14/1/2010 | Cutting session | N/A | Unstained sections. Superfrost slides |
MH006 | ST28 | 19/1/2011 | Cutting session | N/A | poor tissue quality. |
MH007 | ST25 | Only staged has not been cut yet | |||
MH008 | ST19 | 26/01/2011 | Serial Nissl sections | Complete Nissl stained serial sections | |
RC001 | ST22 | 23/11/10 | Histology Training with Satya | Evaluating a protocol to follow to prepare stained serial sections. | |
RC002 | ST23 | 2/12/2010 | Histology Training with Satya | Evaluating a protocol to follow to prepare stained serial sections. | |
RC003 | ST21 | 10/01/2011 | Staging Exercise for Reuben, sectioned by Fabiana, stained by Reuben | Following staining decided to not use H&E staining. | |
RC004 | ST25 | 17/12/2010 | Histology Training with Satya | Some tissue sections good, derived sectioning protocol from these results. | |
RC005 | ST19 | 10/01/2011 | . | ||
RC006 | ST20 | Staged:10/01/2011, Cutting: 13/01/2011 | Sectioned then Nissl stained. | Un-subbed slides were used and the tissues were moving during coverslipping. | |
RC007 | ST19 | 10/01/2011 | Staging Exercise | Staging unconfirmed. | |
RC008 | ST24 | 10/01/2011 | Staging Exercise | Staging unconfirmed. | |
RC009 | ST24 | Sectioned then Nissl stained | Staging unconfirmed, hindbrain not fully cut.Tissue adhering to slide however. | ||
RC010 | ST20 | 10/01/2011 | Staging Exercise | Staging unconfirmed. | |
RC011 | ST26 | 10/01/2011 | Staging Exercise | Staging unconfirmed. | |
RC012 | ST29 | 10/01/2011 | Staging Exercise | Staging unconfirmed. | |
RC013 | ST30 | 10/01/2011 | Staging Exercise | Staging unconfirmed. | |
RC014 | ST31 | 10/01/2011 | Staging Exercise | Staging unconfirmed. | |
RC015 | ST20+ | 10/01/2011 | Staging Exercise | Staging confirmed, embryo sectioned by Malisha, Nissl stained by Reuben, deemed 'good' but not great quality tissue sections by Fabiana. |