Kubke Lab:Research/CND/Records2010-2011Summer/RC008
| Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC008
Embryo stage: ST24
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. (Notebook entry 10th January 2011). Staging has not been confirmed.
- Eye is pigmented (>ST21).
- Leg bud and wing bud are roughly the same size.
- Limb buds are distinctively longer than they are wider.
- Dorsal contour of trunk is curved.
- 2nd Visceral arch distinctly protrudes across the ventral surface of embryo.
Fixation: PFA
Cryoprotection: None.
Material label and storage: Stored in vial filled with PFA labeled RC-008 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Objective: To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST24
Images
Summary
This embryo may be used in further experiments as approximately a ST24 embryo however confirmation is needed.
