Kubke Lab:Research/CND/Records2010-2011Summer/MH004

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Cranial Nerve Development Experiment

Embryo details


Species:Gallus gallus domesticus
Embryo Name:MH004
Embryo stage:ST28-29
Staging description:
Fixation:PF
Cryoprotection:No
Material label and storage:Stored in a vial labelled MH004 filled with PF solution.

Experiment details


Objective: To determine what are the best parameters to cut a stage 28 embryo.
Procedure:

Cryostat Sectioning Cryostat settings (for a more detailed protocol visit Kubke_Lab:Cryomicrotomy)

Cryostat Histology Lab
Knife
Day Cut 16/12/2010
Knife Angle 0
Chamber Temp -16C
Object Temp -16c
Glass Slides Polysine slides
Plane of section Coronal
Number of slides
Observations Fabiana's notes

(Include in your observations, eg ,were the sections serial, was any section lost, was quality assessed, etc)




Comments: Kubke_Lab:/Notebook/Cranial_nerve_development/2010/12/16#Cryostat_sectioning

Results

  • Nissl stained

Images

Summary