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Towards Self-differentiated Bacterial Assembly Line

Our projects concern with the ability for bacterial cells to differentiate out of homogeneous conditions into populations with the division of labor. We aim at devices conferring host cells with the ability to form cooperating groups spontaneously and to take consecutive steps sequentially even when the genetic background and environmental inputs are identical. To break the mirror in such homogeneous condition, we need two devices respectively responsible for temporal and spatial differentiation. The implementation and application of such devices will lead to a picture of future bioengineering where complex programs consisted of sequential steps (structure oriented programs) and cooperating agencies (forked instances of a single class, object and event oriented) can be embedded in a single genome.

About the name of projects, the two devices are named after their respective functions: hop count and push-on-push-off switch, but they are common for breaking symmetry, which is interesting and ubiquitous in science. Although this "differentiation" process resemble the development of multicellular organism, we tend to use a more bioengineering style analogy: assembly line. Or maybe after some years from now, this will not be just an analogy.
Also visit: IGEM2007 Peking

IGEM 2007 Peking Peking The Projects Peking The Team Peking's Misc & Fun Acknowledgement

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Hop Count with Conjugation
Project Proposal
Project Presentation
Road Map
Related Articles

Push-on-push-off Switch
Project proposal
Project presentation
Road Map
Related Articles


Some of our ideas are listed here

Rap up
Collections of the experiences.

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Products and Parts

Hop-count with Conjugation
Push-on-push-off Switch

Count:Agarose Gel Electrophoresis
Count:DNA Gel Extraction
Count:PCR product purifcation
Count:Preparation of Competent Cells
Enzymatic DNA Digestion
Count: Miniprep
Count: Conjugation test
Count: Gene Knock Out
Switch: Miller Method
Switch: PCR
Switch: DNA electrophoresis
Switch: DNA ligation
Switch: DNA digestion
Switch: transformation
Switch: DNA rescue by precipitation
Switch: Plasmid mini prep

Peking 2007 Notebook icon.jpgNotebooks
Hop-count with Conjugation
Push-on-push-off Switch
Turtle Diary

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The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology.




The Team

Our Campus

The Labs

Email us at igem-pku AT googlegroups DOT com

External links iGEM | iGEM 2006 | Registry of Standard Biological Parts | iGEM PKU Googlegroup | iGEM OWW Page