|
|
Constructs Overview
Here are several tables listing the bricks which we are cloning, constructing and using, with their relevant details.
A new page is currently being developed!
Cloning Table
Status of biobricks cloned and their applications:
| I
|
ID
|
Part
|
Description
|
Status
|
Ligation Target
|
Use in Constructs
|
|
02 |
BBa_I13522 |
ptet-GFP |
XL1, Maxi, Mini, BL21 |
--- |
Basic construct for chassis characterization
|
|
11 |
BBa_E7104 |
pT7 GFP |
XL1, Maxi, Midi, Mini, BL21 |
--- |
CBD test construct (pT7)
|
|
30 |
--- |
pT7 (strong GFP) |
XL1, Maxi, Mini, BL21 |
--- |
T7 test-construct
|
| I |
18 |
BBa_T9002 |
ptet-luxR-plux-GFP |
XL1, Maxi, Midi, BL21 |
--- |
Basic construct for chassis characterization
|
|
|
|
|
|
|
|
|
05 |
BBa_R0040 |
ptet |
XL1, Midi, Mini |
DsRedEx |
CBD final (ptet), ID AHL sender
|
|
|
BBa_I719??? |
pT7 |
Ready |
DsRedEx |
CBD final construct (pT7)
|
|
07 |
BBa_F2620 |
ptet-luxR-plux |
XL1, Midi, Mini |
DsRedEx |
ID final construct
|
|
|
|
|
|
|
|
|
21 |
BBa_J23039 |
ptet-luxI |
XL1, Midi |
--- |
ID sender construct
|
|
24 |
BBa_R0062 |
plux |
XL1, Midi |
DsRedEx |
ID construct 2
|
|
25 |
BBa_J37032 |
plux-GFP |
XL1, Maxi, Midi |
--- |
ID construct 2
|
|
26 |
BBa_S03119 |
ptet-luxR |
XL1, Midi |
--- |
---
|
|
|
|
|
|
|
|
|
27 |
pSB1A3 |
Empty pSB1A3 |
Problematic |
--- |
---
|
|
|
|
|
|
|
|
|
13 |
BBa_B0015 |
Double terminator |
XL1, Maxi, Midi, Mini |
--- |
General part construction
|
|
17 |
BBa_I13507 |
RFP |
XL1, Midi |
--- |
Not being used anymore
|
|
19 |
BBa_B0034 |
strong RBS |
XL1 |
--- |
General part construction
|
|
23 |
BBa_I13504 |
GFP |
XL1, Maxi, Midi |
pT7 |
General part construction
|
|
|
|
|
|
|
|
|
|
BBa_I719??? |
DsRed Express |
Ordered |
ptet, plux, pT7 |
Reporter system for ID, Hrp
|
|
|
BBa_I719??? |
HrpR-HrpS |
Ordered |
??? |
Hrp regulators
|
|
|
BBa_I719??? |
pHrpL |
Ordered |
??? |
Hrp promoter
|
|
|
BBa_I719??? |
HrpV |
Ordered |
??? |
Hrp inhibitor system
|
|
|
|
|
|
|
|
Ligation Table
Status of parts ligated and their applications:
| I
|
ID
|
Part
|
Description
|
Status
|
Parents
|
Application
|
|
|
BBa_I719??? |
pT7 & GFP |
Planned |
pT7 + BBa_I13504 |
CBD test construct (pT7)
|
|
|
|
|
|
|
|
|
|
BBa_I719??? |
DsRedEx & pSB1A3 |
Paused |
DsRedEx + BBa_P1010 |
Biobrick Submission
|
|
|
|
|
|
|
|
|
|
BBa_I719??? |
pT7 & DsRedEx |
Planned |
pT7 + DsRedEx |
CBD final construct (pT7)
|
|
|
BBa_I719??? |
ptet & DsRedEx |
Planned |
BBa_R0040 + DsRedEx |
CBD final construct (ptet)
|
|
|
BBa_I719??? |
ptet-luxR-plux-DsRedEx |
Planned |
BBa_F2620 + DsRedEx |
ID detector final construct 1
|
|
|
BBa_I719??? |
plux-DsRedEx |
Planned |
BBa_R0062 + DsRedEx |
ID detector final construct 2
|
|
|
|
|
|
|
|
Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Maxi - maxiprepped; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3); status - stocks running low]
Parts from the Registry
Physical information of biobricks:
| A
|
W
|
G
|
S
|
ID
|
Name
|
Description
|
Plasmid
|
Resist
|
Copy
|
Size(B)
|
Size(T)
|
Location
|
Conc.
(ng/ul)
|
| A |
W |
G |
|
02 |
BBa_I13522 |
GFP under tetracyclin promoter |
pSB1A2 |
AmpR |
High |
937bp |
3016bp |
Well 15H, Plate 2 |
500
|
| A |
W |
G |
|
05 |
BBa_R0040 |
tetracyclin promoter |
pSB1A2 |
AmpR |
High |
54bp |
2133bp |
Well 7O, Plate 1 |
60
|
| A |
W |
G |
|
07 |
BBa_F2620 |
lux promoter, with luxR |
pSB1A2 |
AmpR |
High |
1061bp |
3140bp |
Well 6B, Plate 1 |
80
|
| A |
X |
G |
|
11 |
BBa_E7104 |
T7 GFP |
pSB1A2 |
AmpR |
High |
826bp |
2905bp |
Well 13F, Plate 2 |
100
|
| A |
W |
G |
|
13 |
BBa_B0015 |
Double terminator |
pSB1AK3 |
A+KR |
High |
129bp |
3316bp |
Well 1B, Plate 2 |
100/70
|
| A |
|
G |
|
17 |
BBa_I13507 |
RFP |
pSB1A2 |
AmpR |
High |
861bp |
2940bp |
Well 16N, Plate 1 |
80
|
| A |
W |
G |
|
18 |
BBa_T9002 |
GFP under lux promoter with LuxR |
pSB1A3 |
AmpR |
High |
1945bp |
4102bp |
Well 19B, Plate 2 |
460
|
| A |
W |
|
|
19 |
BBa_B0034 |
RBS |
pSB1A2 |
AmpR |
High |
12bp |
2091bp |
Well 3O, Plate 1 |
--
|
| A |
|
G |
|
21 |
BBa_J23039 |
Constitutive luxI |
pSB1A2 |
AmpR |
High |
860bp |
2939bp |
Well 11K, Plate 3 |
140
|
| A |
W |
G |
|
23 |
BBa_I13504 |
GFP |
pSB1A2 |
AmpR |
High |
875bp |
2954bp |
Well 16E, Plate 1 |
120/330
|
| A |
|
G |
|
24 |
BBa_R0062 |
lux promoter |
pSB1A2 |
AmpR |
High |
55bp |
2134bp |
Well 9G, Plate 1 |
90
|
| A |
|
G |
|
25 |
BBa_J37032 |
GFP under lux promoter |
pSB1A2 |
AmpR |
High |
938bp |
3017bp |
Well 8D, Plate 3 |
125/330
|
| A |
|
G |
|
26 |
BBa_S03119 |
constitutive LuxR |
pSB1A2 |
AmpR |
High |
998bp |
3077bp |
Well 19J, Plate 1 |
60
|
| A |
W |
X |
|
27 |
pSB1A3 |
pSB1A3 (blank) |
pSB1A3 |
Amp |
High |
0bp |
2157bp |
Well 23E, Plate 1 |
--
|
|
W |
|
|
30 |
--- |
T7 with GFP(strong) |
--- |
Kan? |
??? |
--- |
??? |
--- |
150
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Our Parts
Physical information of our ligated parts:
| A
|
W
|
G
|
S
|
ID
|
Name
|
Description
|
Plasmid
|
Resist
|
Copy
|
Size(B)
|
Size(P)
|
Stock
|
|
|
|
|
|
BBa_I719022 |
cI with GFP |
pSB1A2 |
AmpR |
High |
933bp |
3988bp |
|
|
|
|
|
|
BBa_I791021 |
luxI coding region under ptet |
pSB1A2 |
AmpR |
High |
860bp |
2939bp |
|
|
|
|
|
|
BBa_I791??? |
DsRed Express under ptet |
pSB1A2 |
AmpR |
High |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Legend: A (Available), W (Working) [W - working in vivo, W - working in vitro, X - faulty], G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced)
Concentrations are given in ng/μl
Part Graveyard
General information on parts we do not like...
| A
|
W
|
ID
|
Name
|
Description
|
Plasmid
|
Size
|
Status
|
Notes
|
| A |
W |
01 |
BBa_E0040 |
GFP |
pSB1A2 |
720bp |
XL1, Mini |
Redundant
|
| A |
|
03 |
BBa_J5528 |
pBad-GFP |
pSB2K3 |
2093bp |
failed-XL1 |
ara construct abandoned
|
| A |
|
04 |
BBa_J24677 |
plux-GFP |
pSB1A2 |
1787bp |
XL1, Mini |
Wrong GFP ligated
|
| A |
|
06 |
BBa_I0500 |
pBad |
pSB2K3 |
1210bp |
XL1, Mini, Midi |
ara construct abandoned
|
| A |
W |
08 |
BBa_F1610 |
luxI |
pSB1AK3 |
798bp |
XL1, Mini, Midi |
Substitute part J23039
|
| A |
W |
09 |
BBa_R0010 |
lacI-plac |
pSB1A2 |
200bp |
XL1, Mini |
Lac construct abandoned
|
| A |
W |
10 |
BBa_E1010 |
RFP |
pSB2K3 |
681bp |
XL1, Mini |
Substitute part I13507
|
| A |
|
12 |
BBa_S03550 |
araC |
pSB1AC3 |
958bp |
XL1, Mini, Midi |
araC construct abandoned
|
|
W |
14 |
BBa_R0051 |
pcI |
pSB1A2 |
49bp |
Biobrick |
pcI construct abandoned
|
| A |
|
15 |
BBa_J07037 |
cI-GFP |
pSB2K3 |
912bp |
XL1, Mini |
No RBS present on this part
|
| A |
W |
16 |
BBa_E0240 |
GFP |
pSB1A2 |
876bp |
XL1, Midi |
Redundant
|
|
W |
20 |
--- |
AraC |
pSB1AC3 |
1095bp |
Planned |
araC construct abandoned
|
| A |
W |
28 |
BBa_P1010 |
pSB1A3 (ccd) |
pSB1A3 |
0bp |
failed-XL1 |
Strain not available
|
| A |
W |
29 |
BBa_P1010 |
pSB1A2 (ccd) |
pSB1A2 |
0bp |
--- |
Strain not available
|
|
|
|
|
|
|
|
|
|
Construct Diagrams
The original (old) diagrams page was moved to old construct diagrams page. This page also contains the obsolete parts.
CBD Constructs
ptet GFP
pT7 GFP
ptet DsRed express
- Availability: Requires ligation
- Promoter can be found in registry as: BBa_R0040
- DsRed express is to be ordered from GeneArt
pT7 DsRed express
- Availability: Requires ligation
- Promoter is being PCRed from plasmid derived externally
- DsRed express is to be ordered from GeneArt
ID Constructs
pLux GFP with LuxR
 Ptet promoting LuxR, Plux promoting GFP
pLux GFP with LuxR
<<Two more constructs with DsRed express yet to be included>>
|
