IGEM:IMPERIAL/2007/DNA Constructs/Con Diagrams old

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Here lies the ruins of the ancient constructs page

  • Construct CBD 1
    • Entire construct is currently available in Biobricks Registry: BBa_E7104 (available), in pSB1A2 (AmpR)
    • pT7 can be used with T7 RNAP in vitro and in veso, and it gives constitutive expression.
PT7 promoting GFP

  • Construct CBD 2
    • Entire construct is currently in Biobricks Registry: BBa_I13522 (available), in pSB1A2 (AmpR)
    • pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
Ptet promoting GFP

  • Construct CBD 3
    • pBAD promoter with GFP is currently available in Biobricks Registry: BBa_J5528 (available), in pSB2K3 (KanR)
    • Need Ara C regulator protein in vitro and in veso. Ara C available with the RBS:BBa_S03550
    • pBad can be used with E.coli RNAP in vitro, and it is inducible by arabinose.
    • To be used in veso, arabinose transport proteins have to be inserted in the phospholipid bilayer of the vesicles.
PBAD promoting GFP and Ptet promoting araC

  • Construct CBD 4
    • Entire construct is currently available in Biobricks Registry: BBa_J07037 (avaiable), in pSB2K3 (KanR)
    • pcI gives constitutive expression.
PcI promoting GFP

PBAD promoting GFP and PcI promoting araC

  • Construct CBD 6
    • T7 promoter is in the process of cloning
    • dsRed has been ordered
pT7 promoting dsRed

  • Construct CBD 7
    • tet promoter available in the registry: BBa_R0040
    • dsRed has been ordered
Ptet promoting dsRed


  • Construct CBD 8
PBAD promoting RFP and Ptet promoting araC


  • Construct CBD 9
    • λcI regulated promoter available in the registry:BBa_R0051
pcI promoting RFP


  • Construct CBD 10
    • Parts avaiable ....
PBAD promoting RFP and PcI promoting araC


Constructs for Infector Detector

  • Construct ID 1
    • Entire part minus promoter is currently in Biobricks Registry: BBa_F1610 (available), in pSB1AK3 (AmpR, KanR)
    • pTet is currently in Biobricks Registry: BBa_R0040 (available), in pSB1A2 (AmpR)
    • pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
Ptet promoting LuxI

  • Construct ID 2
    • Entire part is currently in Biobricks Registry: BBa_J24677 (available), in pSB1A2 (AmpR)
    • Entire part minus GFP reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
    • GFP reporter system is currently in Biobricks Registry: BBa_I13504 (available)
    • pLux can be used with E.coli RNAP in vitro and maybe in veso, and it is repressed by LuxR but activated by AHL:LuxR complex.
Ptet promoting LuxR, Plux promoting GFP

  • Construct ID 3
    • Entire part minus Hrp system is currently in Biobricks Registry: BBa_F2620(available) in pSB1A2 (AmpR)
    • pHrpL can be used with E.coli RNAP in vitro and maybe in veso, and it is inducible by HrpR:HrpS:Sigma 54 complex.
Ptet promoting LuxR, Plux promoting HrpR and HrpS and PHrpL promoting GFP

  • Construct ID 4
    • Entire part minus dsRed reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
    • dsRed has been ordered
Ptet promoting LuxI

  • Construct ID 5
    • Entire part minus the hrp system and dsRed reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
    • dsRed has been ordered
    • hrpR-hrpS-pHrpL has been ordered
Ptet promoting LuxR, Plux promoting HrpR and HrpS and PHrpL promoting lacZ

This section contains irrelevant data and should be disregarded:
|- style="background:#CCFFCC" align="center" || || || BBa_I719??? || hrpR-hrpS & phrpL || Pending || hrpR-hrpS + phrpL || ID detector test construct 3 and final construct 5 |- style="background:#CCFFCC" align="center" || || || BBa_I719??? || Ptet-luxR-Plux & hrpR-hrpS-phrpL || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] + hrpR-hrpS-phrpL || ID detector test construct 3 and final construct 5 |- style="background:#CCFFCC" align="center" || || || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & GFP || Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || ID detector test construct 3 |- style="background:#CCFFCC" align="center" || || || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & DsRed|| Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + DsRed || ID detector final construct 5