IGEM:IMPERIAL/2007/Projects/Shopping Lists

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<html><h3>THE IMPERIAL iGEM 2007 Team </h3></html>
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Home Cell By Date Infector Detector Wet Lab Dry Lab Resources Meet the Team Entertainment Acknowledgments


Lab Notebook
Daily Record of Wet Lab

Protocols
Fabrication & Testing Protocols

DNA Constructs
Overview of DNA Constructs

Shopping List
Expenditures on Materials


Shopping List

Date Item Order No. Quantity Price Total Cost
1 01/08 Shipping 2039605 17
2 01/08 HiSpeed Plasmid Midi Kit 2039605 25 157.25
3 01/08 QIAquick Gel Extractino Kit 2039605 50 58.65
4 01/08 QIAprep Spin Miniprep Kit 2039605 50 47.85
5 01/08 Kanamycin Monosulfate from Streptomyces 2039606 50.5
6 01/08 Ampicillin Sodium Crystalline 2039606 65.44
7 01/08 Nutrient broth for Microbiology 2039607 500g 50
8 01/08 Cryobox Polycarbonate Yellow Grid 133mmx133mmx51mm 2039608 2ml 29.51
9 01/08 Cryobox Polycarbonate Red Grid 133mmx133mmx51mm 2039608 2ml 29.51
10 01/08 Gene Pulser/Micropulser Cuvettes 0.1cm Gap 2039609 126.35
11 01/08 Delivery charge 2039609 12.5
12 01/08 EcoR1 2039610 34
13 01/08 Xba1 2039610 33
14 01/08 Spe1 2039610 34
15 01/08 Pst1 2039610 42
16 01/08 T4 DNA Ligase 2039610 48 835.56
17 04/08 SYBR Safe DNA gel stain 10 000X concentrate in DMSO 2040668 36.1
18 04/08 N-(-B-Ketocaproyl)-L-Homoserine Lactone 2040669 29.61 901.27
19 09/08 L-Phosphatidic acid sodium from Egg Yolk 2042549 1 10.92
20 09/08 Mineral Oil Molecular Biology Reagent 2042549 500ml 18.35
21 09/08 1,2-Dioleoyl-Sn-glycero-3-phosphocholine 2042549 5ml 53.70
22 09/08 L-Amino Acids Kit 2042549 1 203
23 09/08 T7 RNA Polymerase Recombinant; Expressed 2042549 10mg/ml 29.9
24 09/08 Adenosine 5-Triphosphate Standard *Disodium 2042549 5vl 63.82
25 09/08 Phospho(enol)pyruvis acid monopotassium 2042549 1g 141.07
26 09/08 Pyruvate Kinase Tpe VIII from Bacillus 2042549 1 135.51
27 09/08 Isopropyl B-D-Thiogalactopyranoside *Dioxane 2042549 5g 121.50
28 09/08 Magnesium Acetate Solution, for Molecular Biology 2042549 500ml 120
29 09/08 L-Glutamic Acid Monopotassium 2042549 500g 66.88
30 09/08 YT Microbial Medium 2042549 250g 22.70 1888.62
31 09/08 Geneart 125.09 2013.71
32 14/08 E.coli S30 Extract System for Circular DNA (Promega) L1020 30 reactions 139
33 14/08 E.coli T7 S30 Extract System for Circular DNA (Promega) L1130 30 reactions 139
34 14/08 Nuclease free water (Promega) - 2 x 25µL 21
35 14/08 Delivery charges - - 15 2202.62
36 14/08 Red Filter - 1 140
37 14/08 Red Filter - 1 198
38 14/08 Delivery charges - - 10 2550.62
39 17/08 Oligos(MWG) - - 15.64
40 17/08 Sealing mats(Fischer) - - 16.00
41 21/08 ATP A7699 1g 38.60
42 21/08 CTP 30320 100mg 34.70
43 21/08 GTP G8877 25mg 17.70
44 21/08 UTP 94370 250mg 23.70
45 21/08 E.coli tRNA R4251 500UN 52.60
46 21/08 cAMP 01895 100mg 13.40
47 21/08 Folinic acid F8259 25mg 21.00 2783.96
48 28/8 Recombinant GFP (Clontech) QV071299 1 UN 221.25 (25% discount)
49 28/8 Recombinant DsRED Express (Clontech) QV071299 1 UN 224.25 (25% discount)
50 28/8 Delivery for Clontech - - 30
51 28/8 Qiagen Maxi Kit Cat no.12162 1 107.10
52 28/8 Delivery Charges - 12.00
53 28/8 L-A-Phosphatidyl Choline B Palmitoyl-*GA - 1 UN 140.50
54 28/8 Sorbitan Monooleate - 1 UN 10.08
55 28/8 Dodecane. Anhydrous, 99+ - 1 UN 28.60 3557.74
56 29/8 E.coli S30 Extract System for Circular DNA (Promega) L1020 1 UN 139.00
57 29/8 Delivery charges - - 15 3711.74
58 03/09 E.coli S30 Extract System for Circular DNA (Promega) L1020 3 UN 417.00
59 03/09 Delivery Charges - - 15
60 03/09 Dodecane - 500ml 84.00
61 03/09 Delivery - - 13.00 4240.74
62 04/10 Sequencing of DNA constructs - - 170.00
63 04/10 E.coli S30 Extract System for Circular DNA (Promega) L1020 2 UN 278.00

Acknowledgements

  1. Geneart is giving us 10000 euros worth of products.
  2. Promega gave us a 50% discount for the all of the E.coli S30 extract system and E.coli T7 S30 extract system for circular DNA we have purchsed.
  3. Clontech is giving us 25% discount for all our purchases made.