IGEM:Harvard/2010/General Protocols
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Synthetic Biology
- How Do BioBricks Work?
- Strategy for Making Parts
- PCR
- Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- DNA Gel Electrophoresis and Fragment Purification
- Measuring DNA Concentration Using a Nanodrop Spectrophotometer
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
- Qiagen RNeasy Plant Mini Kit
- iScript cDNA Synthesis
Microbiology
- Growing E. coli
- Growing Agrobacterium
- Making Electro-Competent Agrobacterium
- Electroporating Agrobacterium
