• Devices used for generating ~ 20µm double-emulsion droplets that can be used for Flow cytometry analysis or FACS.
• The microfluidic chip has three inlets: one for the cells, one for the substrate and one for the oil+surfactant.
Tauzin, A.S., Pereira, M.R., Van Vliet, L.D. et al. Investigating host-microbiome interactions by droplet based microfluidics. Microbiome 8, 141 (2020). https://doi.org/10.1186/s40168-020-00911-z
Designed by: Martin Fischlechner
20µm triple inlet for the first emulsion: png, dwg
20µm double inlet for the second emulsion:png, dwg
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Flow rates used:
The chip device used to make the second emulsion or Water-in-Oil-in-Water droplets should be treated to be hydrophilic before use. LvV, 2020