• Devices used for generating ~ 20µm double-emulsion droplets that can be used for Flow cytometry analysis or FACS.
• The microfluidic chip has three inlets: one for the cells, one for the substrate and one for the oil+surfactant.
20µm triple inlet for the first emulsion: png, dwg
20µm double inlet for the second emulsion:png, dwg
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Flow rates used:
The chip device used to make the second emulsion or Water-in-Oil-in-Water droplets should be treated to be hydrophilic before use. LvV, 2020