Dual aqueous inlet flow-focusing junction for the generation of ≈ 3 pL droplets. Blue: depth ≈ 12 µm
D. Schnettler Fernández, O. J. Klein, T. S. Kaminski, P.-Y. Colin, F. Hollfelder.
Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase.
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With flow rates of aqueous phase 2 x 50 µL/h for the two aqueous phases and oil 500 µL/h for the oil phase this device generated 3 pL droplets at rates of 0.5–3 kHz. DSF