DataONE

This DataONE OpenWetWare site contains informal notes for several research projects funded through DataONE. DataONE is a collaboration among many partner organizations, and is funded by the US National Science Foundation (NSF) under a Cooperative Agreement.

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Welcome to our open notebook!

This online notebook captures informal research notes for a few of the research projects funded through the NSF's DataONE cyberinfrastructure project.

Our active OWW project pages are listed here: DataONE:Notebook/Summer_2010#Active_project_page

Have a look at the DataONE site for a more comprehensive list of all DataONE projects.

It is early days for this notebook: feedback is very welcome!

NOTE: The research notes posted on this website are available in the spirit of Open Notebook Science. Content describes research in progress, so please interpret notes and results in that context. We are learning: our notes may be incomplete or delayed. We encourage you to contact the "contributing authors" (lower left sidebar) of any given page for complete information.

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Recent changes

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1 June 2023

			23:23		Lee:Lab Members‎‎ 4 changes history +169‎ [Wooin Lee‎ (4×)]
					23:23 (cur | prev) +164‎ Wooin Lee talk contribs (→‎Research rotation/internship students (past))
					23:21 (cur | prev) +26‎ Wooin Lee talk contribs (→‎Graduate students)
					23:20 (cur | prev) +31‎ Wooin Lee talk contribs (→‎Alumni)
					23:19 (cur | prev) −52‎ Wooin Lee talk contribs (→‎Graduate students)

17:46

BioMicroCenter:Pricing‎ diffhist −79‎ Stuart S. Levine talk contribs (→‎SHORT READ LIBRARY - RNA)

			09:04		(Upload log)‎ [Elizabeth Suesca‎ (2×)]
					09:04 Elizabeth Suesca talk contribs uploaded File:Cloroformo.png ‎
					08:25 Elizabeth Suesca talk contribs uploaded File:Acetato.png ‎

31 May 2023

N    14:39

UA Biophysics:Protocols:Carotenoid Extration ESP‎ diffhist +423‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo")

14:24

UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer‎ diffhist +54‎ Elizabeth Suesca talk contribs

14:23

UA Biophysics:Protocols‎ diffhist −7‎ Elizabeth Suesca talk contribs (→‎BUFFERS)

			N    14:22		UA Biophysics:Protocols:Elution Buffer‎‎ 2 changes history +1,055‎ [Elizabeth Suesca‎ (2×)]
					14:22 (cur | prev) +467‎ Elizabeth Suesca talk contribs
			N		14:07 (cur | prev) +588‎ Elizabeth Suesca talk contribs (Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop...")

			N    14:03		UA Biophysics:Protocols:Calcein ESP‎‎ 3 changes history +1,249‎ [Elizabeth Suesca‎ (3×)]
					14:03 (cur | prev) +43‎ Elizabeth Suesca talk contribs (→‎Procedimiento:)
					14:02 (cur | prev) +63‎ Elizabeth Suesca talk contribs
			N		14:00 (cur | prev) +1,143‎ Elizabeth Suesca talk contribs (Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A...")

12:48

BioMicroCenter:QC‎ diffhist −421‎ Stuart S. Levine talk contribs (→‎AGILENT BIOANALYZER 2100)

			08:34		UA Biophysics:Protocols:Calcein‎‎ 2 changes history −751‎ [Elizabeth Suesca‎ (2×)]
					08:34 (cur | prev) +53‎ Elizabeth Suesca talk contribs
					08:33 (cur | prev) −804‎ Elizabeth Suesca talk contribs (Replaced content with " Calcein_ESP<br>") Tag: Replaced

N    08:31

UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP‎ diffhist +1,206‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...")

06:42

UA Biophysics:Protocols:Lipid Extraction‎ diffhist −9‎ Elizabeth Suesca talk contribs

30 May 2023

			23:52		Hexlab‎‎ 2 changes history +900‎ [Ximiao He‎ (2×)]
					23:52 (cur | prev) +440‎ Ximiao He talk contribs
					18:46 (cur | prev) +460‎ Ximiao He talk contribs

16:25

Lee:JC‎ diffhist +7‎ Wooin Lee talk contribs (→‎Schedule)

14:31

Tomlinson:LabMeeting‎ diffhist −726‎ Ryan E. Tomlinson talk contribs

06:44

UA Biophysics:Protocols:Lipid Extraction‎ diffhist +89‎ Elizabeth Suesca talk contribs

29 May 2023

15:53

Applications of 3D Printing‎ diffhist −15‎ Sarah L. Perry talk contribs (→‎Soft Robotics)