X-Lab

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X-Laboratory for Ion-Channel Engineering

(离子-通道工程研究组)

Beijing Advanced Innovation Center for Biomedical Engineering

School of Biological Science and Engineering, Beihang University

School of Engineering Medicine, Beihang University



From Scientific Discovery of Ion Channels to Engineering Design of Bioactuators and Biosensors


Principle Investigator: Xiaodong LIU, Ph.D. (刘晓冬)




Welcome to the X-Lab!


实验室简介 (LAB DESCRIPTION)


离子通道作为“生命系统中的晶体管”,通过调控胞内外离子动态交换等方式密切参与生命活动。本实验室主要关注离子通道及离子信号,尤其是钙离子通道(电压门控型CaV和瞬时受体电位型TRP)以及钙离子信号,深入理解 CaV1及TRPP激励-构象-门控-信号(Stimulus-Structure-Gating-Signaling)间的机制关联。

践行“分子生物医学工程”这一重要学科发展方向和趋势,本实验室秉承(生物医学)工程的理念和方法,致力于通过生物原理以及工程原理将生物元件、外源因子与兴奋性细胞膜相结合以重建/合成离子通道复合体的动态互作/组成及病/生理功能。上述的生物原件包括通道亚基、调制因子、信号蛋白和电解质等;常用的外源因子为小分子化合物、材料/力场、光电器件和计算机/单元等。

离子通道能够感知跨膜电压、离子信号、机械力及电磁辐射等多种模态外源性激励,这些非凡能力令人称奇,然而离子通道又是怎样在亚分子、分子及细胞水平上传感、转换及编码这些激励的能量和信息呢?综合运用电生理、生化、分子生物学及光学成像,以及生物物理建模、工程设计及分析等多种方法,我们以发现离子通道与离子信号的新机制、新现象和重要病生理为己任,同时基于离子通道开拓生物医学工程创新和物质世界新认知。


当前的具体研究方向包括:1)分子/细胞信息处理(感觉信号转导及神经振荡);2)兴奋性细胞的可塑性:“兴奋-转录-突起发生”耦联及钙通道/钙信号;3)利用这些基础研究的新进展,开发离子-通道相关的新工具、新方法和新仪器,催生新的发现,推动新的应用。



Our research has been in the field of ion channels and ionic signaling, mainly focusing on calcium channels (voltage-gated calcium channels CaV and transient receptor potential channels TRP) and calcium signaling. Major interests are on in-depth gating mechanisms in relation with cell signaling (the couplings of Stimulus-Structure-Gating-Signaling) for CaV1 and TRPP channels. We are particularly attracted by the amazing capabilities of the ion-channel system, curious about how exactly the stimuli (i.e., energy/information) are transduced or encoded at atomic (e.g., ions), molecular (e.g., proteins) and cellular (e.g., neurons) levels? The methodologies utilized in the lab include electrophysiology, optical imaging, molecular & structural biology, biophysical modeling, and engineering design & analysis. Our mission is to discover new phenotypes & mechanisms and key pathological relevance, meanwhile to develop de novo tools/methods based on our scientific discoveries.

Exemplary projects currently being pursued by students/postdocs include:


1) molecular/cellular information processing by ion-channel system such as electrical/calcium oscillations in neurons;


2)plasticity of excitable cells, e.g., excitation-transcription-neuritogenesis coupling mediated by calcium channels and calcium signals


3)de novo tool, methods and instruments based on scientific discoveries on ion-channels, expected to foster new discoveries and applications.














Reprework X2018 GCaMP-X.JPG

Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP



F1000 Recommended!


NOW GCaMP6-X plasmid is available through addgene!

pEGFP-N1-GCaMP6m-XC , ID: 111543.

pEGFP-N1-GCaMP6m-XN , ID: 118976.

AAV-syn-GCaMP6m-XC , ID: 118975.



现在GCaMP6-X质粒可通过北京中原公司(addgene代理)获取!


相关病毒rAAV-hSyn-GCaMP6m-XC-WPRE-hGH polyA (产品编号 PT-1464)!


GCaMP-X 2020 Sum.JPG

GCaMP-X相关质粒全球索取情况 (不完全统计,2020.2更新)


Global requests of GCaMP-X plasmids (incomplete summary,updated 2020.2)























高蕴溟、黎文祥同学合作展示的工作获得第五届全国青年离子通道会议优秀墙报奖 2021.4


X-Lab杨亚雄博士(直博PhD 12-18, 博后Postdoc 18-20)获聘北航生物与医学工程学院准聘副教授,祝贺! 2020.11


左佳琪同学在X-Lab完成的本科毕设工作评为北航生医学院2020优秀毕业论文 2020.6


X-LAB获得北京市自然科学基金资助: 重点(医学 刘晓冬)及 青年(生物科学 杨亚雄)各一项 2020.1


X-LAB获得国家自然科学基金资助:面上(医学部,刘晓冬)及 青年(数理学部,杨亚雄)各一项 2019.8


X-LAB博士生获得国际离子通道大会(7th IICC, Hangzhou China) excellent poster award 2019.6


黎文祥同学在X-Lab完成的本科毕设工作评为北航生医学院2019优秀毕业论文 2019.6


X-LAB博士后杨亚雄获得中国博士后基金第64批面上资助 2018.11 公示名单


X-LAB博士生获国际钙信号与细胞功能(12th SCSC)会议 Best poster award 2018.7


X-LAB获清华大学优秀博士学位论文奖 2018.7


X-LAB博士生杨亚雄顺利通过博士论文答辩,并入选“中国博士后创新人才支持计划”,祝贺! 2018.5 2018博新计划公示名单


X-LAB杨亚雄、刘楠、何元源合作完成的工作在线发表于《Nature Communications》2018.4 Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP Tsinghua Med News


X-LAB与清华大学结构生物学中心研究人员合作完成的工作在线发表于《Nature Communications》2018.3 Cryo-EM structure of the polycystic kidneydisease-like channel PKD2L1 Tsinghua Med News


X-LAB博士生刘楠(直博PhD 11-17)顺利通过博士论文答辩,并即将赴云南大学生命学院任教,祝贺! 2017.6


X-LAB博士生刘楠、杨亚雄、葛琳合作完成的工作在线发表于《eLife》2017.1 Cooperative and acute inhibition by multiple C-terminal motifs of L-type Ca2+ channels Tsinghua NEWS