Construction Files
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University of California Berkeley iGem 2009 Wetlab Team |
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Gene Synthesis of B09ig1 {<azo1653 AtD>}
Pool O09ig1 through O09ig26, assemble by PCA
PCR O09ig1/O09ig26 on PCA reaction (860, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig1 {<azo1653 AtD>}
----
O09ig1 PCA assembly of {<azo1653 AtD>} (B09ig1)
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig2 PCA assembly of {<azo1653 AtD>} (B09ig1)
TAGCACCAGCGTTACCGTCGTCGTTCATACGCTGAGTGCCAGAAGACATCTGG
O09ig3 PCA assembly of {?azo1653 AtD>} (B09ig1)
CGACGGTAACGCTGGTGCTACCACCCAGGACTTCGACGGTGCTCTGATCGGTG
O09ig4 PCA assembly of {?azo1653 AtD?} (B09ig1)
CAACACGCCAGTCACCGAAAGCCGGCAGGTCACCACCGATCAGAGCACCGTCG
O09ig5 PCA assembly of {?azo1653 AtD?} (B09ig1)
TTTCGGTGACTGGCGTGTTGGTGCTGCTATCGGTTACGACATCGCTCAGGTTT
O09ig6 PCA assembly of {?azo1653 AtD?} (B09ig1)
TGGTTTCACCCTCTGCCTGACGAGAACCAACAGAAACCTGAGCGATGTCGTAA
O09ig7 PCA assembly of {?azo1653 AtD?} (B09ig1)
TCAGGCAGAGGGTGAAACCAAAACCCGTCGTTTCGCTCTGTACTCTGGTAAAT
O09ig8 PCA assembly of {?azo1653 AtD?} (B09ig1)
CACGCAGAGAGTTACCGTCAGCCAGTTCGAAAGATTTACCAGAGTACAGAGCG
O09ig9 PCA assembly of {?azo1653 AtD?} (B09ig1)
TGACGGTAACTCTCTGCGTGTTTTCGGTGGTGCTGCTTACTCTCTGCACCGTC
O09ig10 PCA assembly of {?azo1653 AtD?} (B09ig1)
CGTCGATCAGAGAAACGTCACGAGCAGATTCCAGACGGTGCAGAGAGTAAGCA
O09ig11 PCA assembly of {?azo1653 AtD?} (B09ig1)
TGACGTTTCTCTGATCGACGGTGTTGAACACCTGACCCGTGGTTACGACGTTG
O09ig12 PCA assembly of {?azo1653 AtD?} (B09ig1)
GGTAAGCCAGTTCACCGAAGAGTTGACGGGTAACAACGTCGTAACCACGGGTC
O09ig13 PCA assembly of {?azo1653 AtD?} (B09ig1)
CTTCGGTGAACTGGCTTACCGTCTGGGTCTGGGTGAAAAATCTCACGTTGAAC
O09ig14 PCA assembly of {?azo1653 AtD?} (B09ig1)
CACGCTGAGCAACACGCATCAGACCAACGAACGGTTCAACGTGAGATTTTTCA
O09ig15 PCA assembly of {?azo1653 AtD?} (B09ig1)
GATGCGTGTTGCTCAGCGTGCTGACGGTTTCCAGGAAGAAGGTGGTTCTGCTG
O09ig16 PCA assembly of {?azo1653 AtD?} (B09ig1)
GCAGAGCGTTACGCTGAGAGTCACCAGCCAGAGCAGCAGAACCACCTTCTTCC
O09ig17 PCA assembly of {?azo1653 AtD?} (B09ig1)
CTCTCAGCGTAACGCTCTGCTGATCTCTACCCTGGGTGCTCGTGGTCAGCACG
O09ig18 PCA assembly of {?azo1653 AtD?} (B09ig1)
TCAGTTCGATGTCCAGACCACCCAGGTTAACAGCGTGCTGACCACGAGCACCC
O09ig19 PCA assembly of {?azo1653 AtD?} (B09ig1)
TGGTCTGGACATCGAACTGAAAGGTTCTGTTACCTGGCGTCACCTGGAAGGTG
O09ig20 PCA assembly of {?azo1653 AtD?} (B09ig1)
CAGACAGACGCAGACCCAGTTCCGGACGAACGTCACCTTCCAGGTGACGCCAG
O09ig21 PCA assembly of {?azo1653 AtD?} (B09ig1)
ACTGGGTCTGCGTCTGTCTGGTGGTGCTCGTTTCACCGTTATGGGCACTGAAC
O09ig22 PCA assembly of {?azo1653 AtD?} (B09ig1)
CGTTCAGGGTCAGCAGCATAGAGTCACGCGGCAGTTCAGTGCCCATAACGGTG
O09ig23 PCA assembly of {?azo1653 AtD?} (B09ig1)
TATGCTGCTGACCCTGAACGCTAACTACGACCTGATGGCTGGTGTTGTTCTGA
O09ig24 PCA assembly of {?azo1653 AtD?} (B09ig1)
CAGAGTCAGAGGTCATAGCGTTAACACCAACGTTCAGAACAACACCAGCCATC
O09ig25 PCA assembly of {?azo1653 AtD?} (B09ig1)
CGCTATGACCTCTGACTCTGGTAACGCTGCTGCTCTGGTTGCTAACCTGCGCT
O09ig26 PCA assembly of {?azo1653 AtD?} (B09ig1)
CGTTAGGATCCGTTCATTTTCCAGCGCAGGTTAGCAACCAGA
Gene Synthesis of B09ig27 {<Nmul_A1566 AtD>}
Pool O09ig27 through O09ig56, assemble by PCA
PCR O09ig27/O09ig56 on PCA reaction (848, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig27 {?Nmul_A1566 AtD?}
----
O09ig27 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
CCATAGAATTCATGAGATCTGCTTTCTGGACCCGTACCTTCGGTTC
O09ig28 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
GTTCATGTCACCAGAACGGTGACCGAAAGAACCGAAGGTACGGGTCCA
O09ig29 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
ACCGTTCTGGTGACATGAACGCTGCTCGTATCAACCGTAACATCGGTG
O09ig30 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
ACCAGGGTGTCACCACCCATGAAGAAACCACCGATGTTACGGTTGATA
O09ig31 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
ATGGGTGGTGACACCCTGGTTGCTGACCTGCTGCGTCTGGGTATCGCT
O09ig32 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
CGTTGAAAGAAGAGTTAGAGTAACCACCAGCGATACCCAGACGCAGCA
O09ig33 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
CTCTAACTCTTCTTTCAACGTTAACGAACGTTTCTCTACTGGCTCTTC
O09ig34 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
ACCGTAAGCGGTAACGTGGTAGTTGTCAGAAGAGCCAGTAGAGAAACG
O09ig35 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
ACCACGTTACCGCTTACGGTGGCACTCGTTGGCACGCTCTGGGTCTGC
O09ig36 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
TCGTGCCAGGTGTAACCACCACCGAAACGCAGACCCAGAGCGTGCCAA
O09ig37 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
GGTGGTTACACCTGGCACGACTTCGAAACCAACCGTAATATTCTGTTT
O09ig38 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
CACCTTTAGCCTGGTTGTTGAAACCTGGAAACAGAATATTACGGTTGG
O09ig39 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
CAACAACCAGGCTAAAGGTGACTACCGTGGTCGTACTGGCCAGGTTTT
O09ig40 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
GAACGGCAGTTCGTAACCCAGTTCACCGAAAACCTGGCCAGTACGACC
O09ig41 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
TGGGTTACGAACTGCCGTTCAAAAACGTTTCTCTGGAACCGTTCGCTG
O09ig42 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
TTGGTAGCCAGGTTAACGTAAGCAACACCAGCGAACGGTTCCAGAGAA
O09ig43 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
TACGTTAACCTGGCTACCAAAGGTTTCCAGGAACGTGGTGGTATCGCT
O09ig44 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
CTTCGTTGTCACGAGAAGAAGACAGAGCAGCGATACCACCACGTTCCT
O09ig45 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
TTCTTCTCGTGACAACGAAGGTGTTACCTACTCTACCGTTGGTATGAA
O09ig46 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
ACCAGCGGTAGAGAACATAGAAGCAGCGTTCATACCAACGGTAGAGTA
O09ig47 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
CTATGTTCTCTACCGCTGGTGGCACTACCACCCGTCTGCGTGGTTCTC
O09ig48 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
ACAGAGTCGAAAGCGTGACGCCAACCCAGAGAACCACGCAGACGGGTG
O09ig49 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
CGTCACGCTTTCGACTCTGTTCCGACCCGTTCTTCTGTTGCTTTCTCT
O09ig50 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
TGCCAGCGATACCGAAAGCAGAACCACCAGAGAAAGCAACAGAAGAAC
O09ig51 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
TGCTTTCGGTATCGCTGGCACTCCGATCGCTAAAGACGCTATGATCAT
O09ig52 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
ACCAACAGAAGCGTCCAGACCAGCACCGATGATCATAGCGTCTTTAGC
O09ig53 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
GTCTGGACGCTTCTGTTGGTAAAAACGCTATCCTGGGTATCGCTTACA
O09ig54 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
TCAACAACGTTAGAGAAAACCTGGCCAGTGTAAGCGATACCCAGGATA
O09ig55 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
GTTTTCTCTAACGTTGTTGACAACGGTGTTCGTGCTAACCTGGACTGG
O09ig56 PCA assembly of {?Nmul_A1566 AtD?} (B09ig27)
CGTTAGGATCCGAAACGCCAGTCCAGGTTAGCACGAA
Gene Synthesis of B09ig57 {<OprF AtD>}
Pool O09ig57 through O09ig78, assemble by PCA
PCR O09ig57/O09ig78 on PCA reaction (710, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig57 {?OprF AtD?}
----
O09ig57 PCA assembly of {?OprF AtD?} (B09ig57)
CCATAGAATTCATGAGATCTGACTCTAAAATGACTGGCGCTTACGGTC
O09ig58 PCA assembly of {?OprF AtD?} (B09ig57)
AGAGGTAGAGTCAGAGATACCTTTTTCGATCTGACCGTAAGCGCCAGTCATT
O09ig59 PCA assembly of {?OprF AtD?} (B09ig57)
GTATCTCTGACTCTACCTCTGTTGGTCTGATGCTGGGTGGTAACAACAACAA
O09ig60 PCA assembly of {?OprF AtD?} (B09ig57)
TACCTTTCAGGTCAGAGGTAGCGATGTCAACTTTGTTGTTGTTACCACCCAG
O09ig61 PCA assembly of {?OprF AtD?} (B09ig57)
TACCTCTGACCTGAAAGGTAACTCTGCTTACCTGGGTGGTTACGTTAAACAC
O09ig62 PCA assembly of {?OprF AtD?} (B09ig57)
ATACCAGCGATACCACGGAAAGCACCTTTGTCGTGTTTAACGTAACCACCCA
O09ig63 PCA assembly of {?OprF AtD?} (B09ig57)
TTCCGTGGTATCGCTGGTATCGGTATCCAGTACTCTGACTGGGACTCTCACC
O09ig64 PCA assembly of {?OprF AtD?} (B09ig57)
GTTTTCTTTGTAAGAGTCAGACATGGTGTTACGGTGAGAGTCCCAGTCAGAG
O09ig65 PCA assembly of {?OprF AtD?} (B09ig57)
CTGACTCTTACAAAGAAAACTACTCTGACCGTGGTCTGAACATCTACGCAGA
O09ig66 PCA assembly of {?OprF AtD?} (B09ig57)
GACCTTCGGTCAGTTCTTTGGTGTATTTACCCTCTGCGTAGATGTTCAGACC
O09ig67 PCA assembly of {?OprF AtD?} (B09ig57)
CAAAGAACTGACCGAAGGTCTGTTCCTGGAACCGAAAGTTGGTGTTTCTTAC
O09ig68 PCA assembly of {?OprF AtD?} (B09ig57)
GATTCGTTGATAGATTCCTGTTTGATGTAGTCGTAAGAAACACCAACTTTCG
O09ig69 PCA assembly of {?OprF AtD?} (B09ig57)
CAGGAATCTATCAACGAATCTAAAGACAAAGCTCTGGCTCTGGAAGTTGACG
O09ig70 PCA assembly of {?OprF AtD?} (B09ig57)
AACGTTACCAGACAGGGTGTCGAAGTCTTTACCGTCAACTTCCAGAGCCAGA
O09ig71 PCA assembly of {?OprF AtD?} (B09ig57)
ACACCCTGTCTGGTAACGTTGGTCTGGACCTGCGTAAAGAAATCGTTACCGA
O09ig72 PCA assembly of {?OprF AtD?} (B09ig57)
TAACACCAGCGGTCAGGTTGTGTTTACCTTTTTCGGTAACGATTTCTTTACG
O09ig73 PCA assembly of {?OprF AtD?} (B09ig57)
CAACCTGACCGCTGGTGTTAACTACACCCGTATCCTGTCTGGTGCTGACGAA
O09ig74 PCA assembly of {?OprF AtD?} (B09ig57)
GAGTTACCACCGAAGTTGCCAGTCAGGTTGTCTTCGTCAGCACCAGACAGGA
O09ig75 PCA assembly of {?OprF AtD?} (B09ig57)
GGCAACTTCGGTGGTAACTCTTTCGATATTCTGGTTCCGCAGAAAACCAAAG
O09ig76 PCA assembly of {?OprF AtD?} (B09ig57)
GATGTCGTATTTGATACCAACAGAAACGTTGTCTTTGGTTTTCTGCGGAACC
O09ig77 PCA assembly of {?OprF AtD?} (B09ig57)
TTGGTATCAAATACGACATCGAACTGGAAACTGGCGTTATGCTGGGTGCTAA
O09ig78 PCA assembly of {?OprF AtD?} (B09ig57)
CGTTAGGATCCGTAAGAAACTTTAGCACCCAGCATAACGCC
Gene Synthesis of B09ig79 {<cl02365 AtD>}
Pool O09ig79 through O09ig112, assemble by PCA
PCR O09ig79/O09ig112 on PCA reaction (854, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig79 {?cl02365 AtD?}
----
O09ig79 PCA assembly of {?cl02365 AtD?} (B09ig79)
CCATAGAATTCATGAGATCTGAAAACTCTAACGCTCGTA
O09ig80 PCA assembly of {?cl02365 AtD?} (B09ig79)
CACTGACCAACGCCAGTCATCCACATACGAGCGTTAGAGTTTTCA
O09ig81 PCA assembly of {?cl02365 AtD?} (B09ig79)
ATGACTGGCGTTGGTCAGTGGGGTAACCTGGACTACGGTTACTCT
O09ig82 PCA assembly of {?cl02365 AtD?} (B09ig79)
CAGCGTAGAAGTCAACGTCCATGTTAGAGTAACCGTAGTCCAGGT
O09ig83 PCA assembly of {?cl02365 AtD?} (B09ig79)
GGACGTTGACTTCTACGCTGGTCTGGTTGGTGCTGAAATCGACGT
O09ig84 PCA assembly of {?cl02365 AtD?} (B09ig79)
CAGACCAGCTTTGGTGTTTTCGGTAACGTCGATTTCAGCACCAAC
O09ig85 PCA assembly of {?cl02365 AtD?} (B09ig79)
AAAACACCAAAGCTGGTCTGTTCTTCGGTGCTGGTTCTACCCACT
O09ig86 PCA assembly of {?cl02365 AtD?} (B09ig79)
ATTTTACCGTATTCGTGAGCTTTGTAGTGGGTAGAACCAGCACCG
O09ig87 PCA assembly of {?cl02365 AtD?} (B09ig79)
GCTCACGAATACGGTAAAATCGACTCTAACGACCTCCATGTTGGT
O09ig88 PCA assembly of {?cl02365 AtD?} (B09ig79)
CTTTGATGTTAGAAACACCGTACAGACCAACATGGAGGTCGTTAG
O09ig89 PCA assembly of {?cl02365 AtD?} (B09ig79)
CGGTGTTTCTAACATCAAAGACGTTGCTGCTGTTAACTACGGTAT
O09ig90 PCA assembly of {?cl02365 AtD?} (B09ig79)
GTCACGGTCCTGGTTGGTGTGGGTGATACCGTAGTTAACAGCAGC
O09ig91 PCA assembly of {?cl02365 AtD?} (B09ig79)
ACACCAACCAGGACCGTGACGCTAAACGTACCCTGTGGGTTGGTC
O09ig92 PCA assembly of {?cl02365 AtD?} (B09ig79)
GAGGTAGAGTTGTAACCAGAGGTCTGACCAACCCACAGGGTACGT
O09ig93 PCA assembly of {?cl02365 AtD?} (B09ig79)
TCTGGTTACAACTCTACCTCTTACGACGCTAAAATCACCCAGTTC
O09ig94 PCA assembly of {?cl02365 AtD?} (B09ig79)
GTTGGGTGTAAGCACCTTCCAGGAAGAACTGGGTGATTTTAGCGT
O09ig95 PCA assembly of {?cl02365 AtD?} (B09ig79)
GGAAGGTGCTTACACCCAACTCAACAACGACAAATACTCTATCGA
O09ig96 PCA assembly of {?cl02365 AtD?} (B09ig79)
GAGCCAGTTGAAACCAGCGTACGGTTCGATAGAGTATTTGTCGTT
O09ig97 PCA assembly of {?cl02365 AtD?} (B09ig79)
ACGCTGGTTTCAACTGGCTCCATGTTTCTACCGACTCTATCAACG
O09ig98 PCA assembly of {?cl02365 AtD?} (B09ig79)
GAGAACTGCATGTTACCAACCTGTTCGTTGATAGAGTCGGTAGAA
O09ig99 PCA assembly of {?cl02365 AtD?} (B09ig79)
GTTGGTAACATGCAGTTCTCTACCAAAACCGACAACCAGGACATC
O09ig100 PCA assembly of {?cl02365 AtD?} (B09ig79)
CACGCAGACCGATAGTGCCAACCTGGATGTCCTGGTTGTCGGTTT
O09ig101 PCA assembly of {?cl02365 AtD?} (B09ig79)
TGGCACTATCGGTCTGCGTGGTGGTTACCCGTTCACCGCTGGTTC
O09ig102 PCA assembly of {?cl02365 AtD?} (B09ig79)
GTCACCTTTCAGAGCCATGTTGATAGAACCAGCGGTGAACGGGTA
O09ig103 PCA assembly of {?cl02365 AtD?} (B09ig79)
ACATGGCTCTGAAAGGTGACGTTTCTGCTTCTCACCTGTTCGGTG
O09ig104 PCA assembly of {?cl02365 AtD?} (B09ig79)
TAGAGTTGAGCTTCCGGACGGTTGTCACCGAACAGGTGAGAAGCA
O09ig105 PCA assembly of {?cl02365 AtD?} (B09ig79)
CGTCCGGAAGCTCAACTCTACCTGTCTAACTCTGGTGAAGCTACC
O09ig106 PCA assembly of {?cl02365 AtD?} (B09ig79)
GGTTGTCCAGTTTACCACCACGCAGGGTAGCTTCACCAGAGTTAG
O09ig107 PCA assembly of {?cl02365 AtD?} (B09ig79)
TGGTGGTAAACTGGACAACCTGTTCGGTGTTGGTCTGGGTGTTGA
O09ig108 PCA assembly of {?cl02365 AtD?} (B09ig79)
GGTGGTAGATTTGTTGAGTTGAGCGTCAACACCCAGACCAACACC
O09ig109 PCA assembly of {?cl02365 AtD?} (B09ig79)
AACTCAACAAATCTACCACCTTCGGTGTTTCTTACCAGGGTCAGT
O09ig110 PCA assembly of {?cl02365 AtD?} (B09ig79)
CCAGAAGAAGAAACGTCAGAGTTGTACTGACCCTGGTAAGAAACA
O09ig111 PCA assembly of {?cl02365 AtD?} (B09ig79)
TCTGACGTTTCTTCTTCTGGTATCAACGCTACCCTGAAAGTTAAC
O09ig112 PCA assembly of {?cl02365 AtD?} (B09ig79)
CGTTAGGATCCGAAGTTAACTTTCAGGGTAGCGT
Gene Synthesis of B09ig113 {<VtaA11 AtD>}
Pool O09ig113 through O09ig120, assemble by PCA
PCR O09ig113/O09ig120 on PCA reaction (269, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig113 {?VtaA11 AtD?}
----
O09ig113 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCATAGAATTCATGAGATCTAACAACAAAGCTCTGCGTGCTGGTATCG
O09ig114 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCTGCGGCAGACCAGCAGCAGCGTTAGAACCAGCGATACCAGCACGCAGAGCT
O09ig115 PCA assembly of {?VtaA11 AtD?} (B09ig113)
TGCTGCTGGTCTGCCGCAGGTTTATATTCCGGGTAAATCTATGGTTGCTGTTT
O09ig116 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCAGAGCAGACTGACCTTTGAAAGTGCCAGCAGAAACAGCAACCATAGATTTA
O09ig117 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CAAAGGTCAGTCTGCTCTGGCTGTTGGTTACTCTCGTGCTTCTGACAACGGTA
O09ig118 PCA assembly of {?VtaA11 AtD?} (B09ig113)
TGTTAGCGTTACCTTGGAGTTTCAGGATCAGTTTACCGTTGTCAGAAGCACGA
O09ig119 PCA assembly of {?VtaA11 AtD?} (B09ig113)
ACTCCAAGGTAACGCTAACACCTCTGGTGAAATGGGTGGTTCTGTTGGTGTTG
O09ig120 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CGTTAGGATCCCCACTGGTAACCAACACCAACAGAACCACCC
Gene Synthesis of B09ig121 {<Hag AtD>}
Pool O09ig121 through O09ig128, assemble by PCA
PCR O09ig121/O09ig128 on PCA reaction (269, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig121 {?Hag AtD?}
----
O09ig121 PCA assembly of {?Hag AtD?} (B09ig121)
CCATAGAATTCATGAGATCTAACGAAAACAAAGCTAACGCTGGTATCT
O09ig122 PCA assembly of {?Hag AtD?} (B09ig121)
CCTGCGGCATAGAAGCCATAGCCATAGCAGAAGAGATACCAGCGTTAGCTTTG
O09ig123 PCA assembly of {?Hag AtD?} (B09ig121)
TATGGCTTCTATGCCGCAGGCTTATATTCCGGGTCGTTCTATGGTTACTGGCG
O09ig124 PCA assembly of {?Hag AtD?} (B09ig121)
CAACAGCACCCTGACCGTTGTGGGTAGCGATACCGCCAGTAACCATAGAACGA
O09ig125 PCA assembly of {?Hag AtD?} (B09ig121)
CAACGGTCAGGGTGCTGTTGCTGTTGGTCTGTCTAAACTGTCTGACAACGGTC
O09ig126 PCA assembly of {?Hag AtD?} (B09ig121)
TGTCAGCAGAACCGTTGATTTTGAAAACCCACTGACCGTTGTCAGACAGTTTA
O09ig127 PCA assembly of {?Hag AtD?} (B09ig121)
AATCAACGGTTCTGCTGACACTCAAGGCCATGTAGGTGCTGCTGTTGGTGCTG
O09ig128 PCA assembly of {?Hag AtD?} (B09ig121)
CGTTAGGATCCGAAGTGGAAACCAGCACCAACAGCAGCACCT
Gene Synthesis of B09ig129 {<Pcryo_1225 AtD>}
Pool O09ig129 through O09ig136, assemble by PCA
PCR O09ig129/O09ig136 on PCA reaction (272, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig129 {?Pcryo_1225 AtD?}
----
O09ig129 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
CCATAGAATTCATGAGATCTGTTGAAGACGACGCTAACGCTGGT
O09ig130 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
GCGGCAGAGAAGACATAGCCATAGCAGCAGAGATACCAGCGTTAGCGTCGTCTT
O09ig131 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
GGCTATGTCTTCTCTGCCGCAGGCTTACATCATCGGTAAACCGATGATCGGTGG
O09ig132 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
AACAGCAGATTCACCGTTGTAGGTAGCGATACCACCACCGATCATCGGTTTACC
O09ig133 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
ACAACGGTGAATCTGCTGTTGCTATCGGTTTCTCTAAAATGTCTAACGACGGTC
O09ig134 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
GTGTCAGCAGTGCCGTTCAGTTTCATAACCCAACGACCGTCGTTAGACATTTTA
O09ig135 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
CTGAACGGCACTGCTGACACTCAAGGCAATGTAGGTGCTGCTATCGGTGCTGGT
O09ig136 PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
CGTTAGGATCCGTCGAAGTGGAAACCAGCACCGATAGCAGCAC
Gene Synthesis of B09ig171 {<gliadin binding scFv>}
Pool O09ig171 through O09ig200, assemble by PCA
PCR O09ig171/O09ig200 on PCA reaction (842, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig171 {?gliadin binding scFv?}
----
O09ig171 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CCATAGAATTCATGAGATCTGCTCAGGTTCAACTCGTTCA
O09ig172 PCA assembly of {?gliadin binding scFv?} (B09ig171)
ACCCGGCTGAACAACACCACCACCAGACTGAACGAGTTGAACCTGAGC
O09ig173 PCA assembly of {?gliadin binding scFv?} (B09ig171)
GTGGTGTTGTTCAGCCGGGTCGTTCTCTGCGTCTGTCTTGCGCTGCTT
O09ig174 PCA assembly of {?gliadin binding scFv?} (B09ig171)
ATATCGTAGATAGAGAAAGAGAAACCAGAAGCAGCGCAAGACAGACGC
O09ig175 PCA assembly of {?gliadin binding scFv?} (B09ig171)
TCTTTCTCTATCTACGATATGTACTGGGTTCGTCAGGCTCCGGGTAAA
O09ig176 PCA assembly of {?gliadin binding scFv?} (B09ig171)
TAGAGATAACAGCAACCCATTCCAGACCTTTACCCGGAGCCTGACGAA
O09ig177 PCA assembly of {?gliadin binding scFv?} (B09ig171)
ATGGGTTGCTGTTATCTCTACCGACGGTTCTAACAAATTCTACGCTGA
O09ig178 PCA assembly of {?gliadin binding scFv?} (B09ig171)
AGAGATGGTGAAACGACCTTTAACAGAGTCAGCGTAGAATTTGTTAGA
O09ig179 PCA assembly of {?gliadin binding scFv?} (B09ig171)
AAGGTCGTTTCACCATCTCTCGTGACAACTCTAAAAACACCCTGTACC
O09ig180 PCA assembly of {?gliadin binding scFv?} (B09ig171)
TCCTCAACACCCAGAGAGTTCATTTGGAGGTACAGGGTGTTTTTAGAG
O09ig181 PCA assembly of {?gliadin binding scFv?} (B09ig171)
AACTCTCTGGGTGTTGAGGATACCGCTGTTTACTACTGCGCTCGTGAA
O09ig182 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CACCCCATTCTTCGTAAGAACCGTCGATTTCACGAGCGCAGTAGTAAA
O09ig183 PCA assembly of {?gliadin binding scFv?} (B09ig171)
TTCTTACGAAGAATGGGGTGACTACTGGGGTCAGGGCACTCTGGTTTC
O09ig184 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CGGAGAGGTTGGGGAAGCAGAAGAAACAGAAACCAGAGTGCCCTGACC
O09ig185 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CTGCTTCCCCAACCTCTCCGAAACTGGAAGAAGGTGAGTTTTCCGAGG
O09ig186 PCA assembly of {?gliadin binding scFv?} (B09ig171)
GGCTGGGTCAGAACAGACTGAACACGAGCCTCGGAAAACTCACCTTCT
O09ig187 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CAGTCTGTTCTGACCCAGCCGCCGTCTGTTTCTGGTGCTCCGGGTCAG
O09ig188 PCA assembly of {?gliadin binding scFv?} (B09ig171)
AAGAGCCAGTGCAAGAGATGGTAACACGCTGACCCGGAGCACCAGAAA
O09ig189 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CATCTCTTGCACTGGCTCTTCTTCTAACATCGGTGCTGGTTACGACGT
O09ig190 PCA assembly of {?gliadin binding scFv?} (B09ig171)
AGTGCCCGGGAGATGTTGATACCAGTGAACGTCGTAACCAGCACCGAT
O09ig191 PCA assembly of {?gliadin binding scFv?} (B09ig171)
ATCAACATCTCCCGGGCACTGCTCCGAAACTGCTGATCTACGGTAACA
O09ig192 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CGGTCCGGAACACCAGACGGACGGTTTTTGTTACCGTAGATCAGCAGT
O09ig193 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CCGTCTGGTGTTCCGGACCGTTTCTCTGGTTCTAAATCTGGCACTTCT
O09ig194 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CTTGGAGGCCAGTGATAGCCAGAGAAGCAGAAGTGCCAGATTTAGAAC
O09ig195 PCA assembly of {?gliadin binding scFv?} (B09ig171)
GGCTATCACTGGCCTCCAAGCTGAGGATGAAGCTGACTACTACTGCGC
O09ig196 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CCAAACGTTCAGAGAGTCGTCCCAAGCAGCGCAGTAGTAGTCAGCTTC
O09ig197 PCA assembly of {?gliadin binding scFv?} (B09ig171)
ACGACTCTCTGAACGTTTGGGTTTTCGGTGGTGGCACTAAACTGACCG
O09ig198 PCA assembly of {?gliadin binding scFv?} (B09ig171)
GACGGAGCAGCTTTCGGCTGACCCAGAACGGTCAGTTTAGTGCCACCA
O09ig199 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CAGCCGAAAGCTGCTCCGTCTGTTACCCTGTTCCCGCCATCCTCTGCT
O09ig200 PCA assembly of {?gliadin binding scFv?} (B09ig171)
CGTTAGGATCCAGCAGCAGCAGAGGATGGCGGGAACA
Gene Synthesis of B09ig201 {<Cub>}
Pool O09ig201 through O09ig206, assemble by PCA
PCR O09ig201/O09ig206 on PCA reaction (164, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig201 {?Cub?}
----
O09ig201 PCA assembly of {?Cub?} (B09ig201)
CCATAGAATTCATGAGATCTTCTGGTGGTATCCCGCCGG
O09ig202 PCA assembly of {?Cub?} (B09ig201)
TACCAGCGAAGATGAGGCGTTGCTGGTCCGGCGGGATACCACCAGAA
O09ig203 PCA assembly of {?Cub?} (B09ig201)
ACGCCTCATCTTCGCTGGTAAACAACTCGAAGACGGTCGTACCCTGT
O09ig204 PCA assembly of {?Cub?} (B09ig201)
TAGATTCTTTCTGAATATTGTAGTCAGACAGGGTACGACCGTCTTCG
O09ig205 PCA assembly of {?Cub?} (B09ig201)
CAATATTCAGAAAGAATCTACCCTCCATCTGGTTCTGCGTCTGCGTG
O09ig206 PCA assembly of {?Cub?} (B09ig201)
CGTTAGGATCCACCACCACGCAGACGCAGAACCAGA
Gene Synthesis of B09ig207 {<Nub>}
Pool O09ig207 through O09ig212, assemble by PCA
PCR O09ig207/O09ig212 on PCA reaction (173, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig207 {?Nub?}
----
O09ig207 PCA assembly of {?Nub?} (B09ig207)
CCATAGAATTCATGAGATCTGGTCTGATGCAAATTTTCGTTAA
O09ig208 PCA assembly of {?Nub?} (B09ig207)
CAGGGTGCCAGTTTTGCCAGTCAGGGTTTTAACGAAAATTTGCATCAG
O09ig209 PCA assembly of {?Nub?} (B09ig207)
CTGGCAAAACTGGCACCCTGGAAGTTGAATCTTCTGACACCATCGACA
O09ig210 PCA assembly of {?Nub?} (B09ig207)
TCTTTGTCCTGGATTTTAGATTTAACGTTGTCGATGGTGTCAGAAGAT
O09ig211 PCA assembly of {?Nub?} (B09ig207)
TCTAAAATCCAGGACAAAGAAGGTATTCCAGGTGGTTGCCGTCCGCGT
O09ig212 PCA assembly of {?Nub?} (B09ig207)
CGTTAGGATCCCATAACACGCGGACGGCAACCACCTG
Gene Synthesis of B09ig137 {<Hia AtD>}
Pool O09ig137 through O09ig144, assemble by PCA
PCR O09ig137/O09ig144 on PCA reaction (269, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig137 {?Hia AtD?}
----
O09ig137 PCA assembly of {?Hia AtD?} (B09ig137)
CCATAGAATTCATGAGATCTCTGCGTCGTGAAACCTCTGGTGGTGTTG
O09ig138 PCA assembly of {?Hia AtD?} (B09ig137)
CCTGCGGCAGGTTAGCGGTAGCGATAGCAGCAGCAACACCACCAGAGGTTTCA
O09ig139 PCA assembly of {?Hia AtD?} (B09ig137)
TACCGCTAACCTGCCGCAGGCTTACGTTCAGGGTCGTGGTATGACCTCTGTTG
O09ig140 PCA assembly of {?Hia AtD?} (B09ig137)
CGATAGCAGACTGACCCTGGTAAGAAGAAACACCAACAGAGGTCATACCACGA
O09ig141 PCA assembly of {?Hia AtD?} (B09ig137)
CCAGGGTCAGTCTGCTATCGCTGTTGGTGTTTCTGCTGTTTCTGAATCTGGTC
O09ig142 PCA assembly of {?Hia AtD?} (B09ig137)
TGTTAGCAGAACCAGAGAATTTGAAAACCCAGTGACCAGATTCAGAAACAGCA
O09ig143 PCA assembly of {?Hia AtD?} (B09ig137)
ATTCTCTGGTTCTGCTAACACCCGTTCTCACGTTGGTGTTGGTGCTGGTGTTG
O09ig144 PCA assembly of {?Hia AtD?} (B09ig137)
CGTTAGGATCCCCATTGATAGCCAACACCAGCACCAACACCA
Gene Synthesis of B09ig145 {<type IIIs Needle Complex scFV>}
Pool O09ig145 through O09ig170, assemble by PCA
PCR O09ig145/O09ig170 on PCA reaction (863, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig145 {?type IIIs Needle Complex scFV?}
----
O09ig145 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CCATAGAATTCATGAGATCTGCTGAACTGGTTATGACCCAGACCCCGT
O09ig146 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
TAACAGTGCCACCAACAGCAGCGGAAACCGGAGACGGGGTCTGGGTCATAACC
O09ig147 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
TGCTGTTGGTGGCACTGTTACCATCTCTTGCCAATCCTCTCAGTCTGTTTACA
O09ig148 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
GTTTCTGCTGATACCAAGACAGCAGGTTGTTGTTGTAAACAGACTGAGAGGAT
O09ig149 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
GTCTTGGTATCAGCAGAAACCGGGTCAGCCGCCGAAACTGCTGATCTACAACG
O09ig150 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
AACGAGACGGAACACCAGAAGCCAGTTTAGAAGCGTTGTAGATCAGCAGTTTC
O09ig151 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
TTCTGGTGTTCCGTCTCGTTTCAAAGGTTCTGGTTCTGGCACTCAGTTCACCC
O09ig152 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CTGCGTCGTCGCATTCGAGATCGGAAATAGTCAGGGTGAACTGAGTGCCAGAA
O09ig153 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
TCTCGAATGCGACGACGCAGCAACCTACTACTGCCAGGGCACTTACTACTCTT
O09ig154 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
TAGTGCCACCACCGAAGGTCAGATACCAACCAGAAGAGTAGTAAGTGCCCTGG
O09ig155 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
GACCTTCGGTGGTGGCACTAAACTGGACGTTAAAGGTCAGTCTGGTCAGTCTG
O09ig156 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CAACACCCTGACCTTCACCAGAACCCGGTTTACCAGACTGACCAGACTGACCT
O09ig157 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
TGGTGAAGGTCAGGGTGTTGGTTCTCAGCAACAACTCGTTGAATCTGGTGGTC
O09ig158 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
GCAGGGTCAGCGGAGTGCCTGGAGTAACCAGACGACCACCAGATTCAACGAGT
O09ig159 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
AGGCACTCCGCTGACCCTGCGTTGCACCGCTTCTGGTTTCTCTCTGTCTAACT
O09ig160 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
TACCCGGAGCCTGACGAACCCAGTTCATGTAGTAGTTAGACAGAGAGAAACCA
O09ig161 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
GGTTCGTCAGGCTCCGGGTAAAGGTCTGGAATGGATCGGTATCATCTACGAAT
O09ig162 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CTTTAGCCCAAGAAGCGTAGTAGGTGAAACCAGATTCGTAGATGATACCGATC
O09ig163 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CTACGCTTCTTGGGCTAAAGGTCGTTTCACCATCTCTAAAACCTCTACCACTA
O09ig164 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CCTCGGTGGTTGGGGAGGTGATTTTCAGGTCCATAGTGGTAGAGGTTTTAGAG
O09ig165 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CACCTCCCCAACCACCGAGGATACCGCTACCTACTTCTGCGCTCGTTTCGACA
O09ig166 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CCCACGGGTCGAAAGCACCACCACGGTAACCGATGTCGAAACGAGCGCAGAAG
O09ig167 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
TGGTGCTTTCGACCCGTGGGGTCCGGGCACTCTGGTTACCGTTTCTTCTGGTC
O09ig168 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CAACGAGTTGAGAAACAGACGGAGCTTTCGGCTGACCAGAAGAAACGGTAACC
O09ig169 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
GTCTGTTTCTCAACTCGTTGGTGCTGAACAAAAGCTCATCTCCGAAGAGGACC
O09ig170 PCA assembly of {?type IIIs Needle Complex scFV?} (B09ig145)
CGTTAGGATCCAGCACCGTTCAGGTCCTCTTCGGAGATGAGC
Gene Synthesis of B09ig213 {<caspase 3>}
Pool O09ig213 through O09ig238, assemble by PCA
PCR O09ig213/O09ig238 on PCA reaction (860, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig213 {?caspase 3?}
----
O09ig213 PCA assembly of {?caspase 3?} (B09ig213)
CCATAGAATTCATGAGATCTGAAAACACCGAAAACTCTGTTGACT
O09ig214 PCA assembly of {?caspase 3?} (B09ig213)
TGATTTTCGGTTCCAGGTTTTTGATAGATTTAGAGTCAACAGAGTTTTCGGTG
O09ig215 PCA assembly of {?caspase 3?} (B09ig213)
AAACCTGGAACCGAAAATCATTCATGGTTCTGAATCTATGGACTCTGGTATCT
O09ig216 PCA assembly of {?caspase 3?} (B09ig213)
CCGGGTAGTCCATTTTGTAAGAGTTGTCCAGAGAGATACCAGAGTCCATAGAT
O09ig217 PCA assembly of {?caspase 3?} (B09ig213)
TTACAAAATGGACTACCCGGAAATGGGTCTGTGCATCATCATCAACAACAAAA
O09ig218 PCA assembly of {?caspase 3?} (B09ig213)
AACGAGAGGTCATGCCAGTGGACTTATGAAAGTTTTTGTTGTTGATGATGATG
O09ig219 PCA assembly of {?caspase 3?} (B09ig213)
CACTGGCATGACCTCTCGTTCTGGCACTGACGTTGACGCTGCTAACCTGCGTG
O09ig220 PCA assembly of {?caspase 3?} (B09ig213)
TACGAACTTCGTATTTCAGGTTACGGAAGGTTTCACGCAGGTTAGCAGCGTCA
O09ig221 PCA assembly of {?caspase 3?} (B09ig213)
CCTGAAATACGAAGTTCGTAACAAAAACGACCTGACCCGTGAAGAAATCGTTG
O09ig222 PCA assembly of {?caspase 3?} (B09ig213)
AGTGATCCTCTTTAGAAACGTCACGCATCAGTTCAACGATTTCTTCACGGGTC
O09ig223 PCA assembly of {?caspase 3?} (B09ig213)
CGTTTCTAAAGAGGATCACTCTAAACGTTCTTCTTTCGTTTGCGTTCTGCTGT
O09ig224 PCA assembly of {?caspase 3?} (B09ig213)
TAGTGCCGAAGATGATACCTTCTTCACCGTGAGACAGCAGAACGCAAACGAAA
O09ig225 PCA assembly of {?caspase 3?} (B09ig213)
AGGTATCATCTTCGGCACTAACGGTCCGGTTGACCTGAAAAAAATCACCAACT
O09ig226 PCA assembly of {?caspase 3?} (B09ig213)
CAGTCAGAGAACGGCAGCGATCGCCGCGAAAGAAGTTGGTGATTTTTTTCAGG
O09ig227 PCA assembly of {?caspase 3?} (B09ig213)
TCGCTGCCGTTCTCTGACTGGCAAACCAAAGCTCTTTATTATTCAGGCTTGTC
O09ig228 PCA assembly of {?caspase 3?} (B09ig213)
CGGTTTCGATACCGCAGTCCAGTTCAGTGCCGCGACAAGCCTGAATAATAAAG
O09ig229 PCA assembly of {?caspase 3?} (B09ig213)
GGACTGCGGTATCGAAACCGACTCTGGTGTTGACGACGACATGGCTTGCCACA
O09ig230 PCA assembly of {?caspase 3?} (B09ig213)
AAGCGTACAGGAAGTCAGCTTCAACCGGGATTTTGTGGCAAGCCATGTCGTCG
O09ig231 PCA assembly of {?caspase 3?} (B09ig213)
AGCTGACTTCCTGTACGCTTACTCTACCGCTCCGGGTTACTACTCTTGGCGTA
O09ig232 PCA assembly of {?caspase 3?} (B09ig213)
GGGATTGAATAAACCAAGAACCGTCTTTAGAGTTACGCCAAGAGTAGTAACCC
O09ig233 PCA assembly of {?caspase 3?} (B09ig213)
TTCTTGGTTTATTCAATCCCTCTGCGCTATGCTGAAACAGTACGCTGACAAAC
O09ig234 PCA assembly of {?caspase 3?} (B09ig213)
GGTTAACACGGGTCAGAATATGCATAAACTCCAGTTTGTCAGCGTACTGTTTC
O09ig235 PCA assembly of {?caspase 3?} (B09ig213)
TATTCTGACCCGTGTTAACCGTAAAGTTGCTACCGAGTTTGAATCTTTCTCTT
O09ig236 PCA assembly of {?caspase 3?} (B09ig213)
GGATCTGTTTTTTAGCGTGGAAGGTAGCGTCGAAAGAGAAAGATTCAAACTCG
O09ig237 PCA assembly of {?caspase 3?} (B09ig213)
CCACGCTAAAAAACAGATCCCGTGCATCGTTTCTATGCTGACCAAAGAACTGT
O09ig238 PCA assembly of {?caspase 3?} (B09ig213)
CGTTAGGATCCGTGGTAGAAGTACAGTTCTTTGGTCAGCATA
Gene Synthesis of B09ig239 {<mgfp-5>}
Pool O09ig239 through O09ig248, assemble by PCA
PCR O09ig239/O09ig248 on PCA reaction (260, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig239 {?mgfp-5?}
----
O09ig239 PCA assembly of {?mgfp-5?} (B09ig239)
CCATAGAATTCATGAGATCTTCTTCCGAGGAATACA
O09ig240 PCA assembly of {?mgfp-5?} (B09ig239)
GGTGTTACCCGGGTAGTAACCACCTTTGTATTCCTCGGAAGAAGAT
O09ig241 PCA assembly of {?mgfp-5?} (B09ig239)
GTTACTACCCGGGTAACACCTACCACTACCACTCTGGTGGTTCTTA
O09ig242 PCA assembly of {?mgfp-5?} (B09ig239)
AACCACCGTGGTAACCAGAACCGTGGTAAGAACCACCAGAGTGGTA
O09ig243 PCA assembly of {?mgfp-5?} (B09ig239)
TTCTGGTTACCACGGTGGTTACAAAGGTAAATACTACGGTAAAGCT
O09ig244 PCA assembly of {?mgfp-5?} (B09ig239)
TTTTTGTATTTGTAGTAGTATTTTTTAGCTTTACCGTAGTATTTAC
O09ig245 PCA assembly of {?mgfp-5?} (B09ig239)
TACTACTACAAATACAAAAACTCTGGTAAATACAAATACCTGAAAA
O09ig246 PCA assembly of {?mgfp-5?} (B09ig239)
ACCTTTACGGTGGTATTTACGAGCTTTTTTCAGGTATTTGTATTTA
O09ig247 PCA assembly of {?mgfp-5?} (B09ig239)
GTAAATACCACCGTAAAGGTTACAAAAAATACTACGGTGGTGGTTC
O09ig248 PCA assembly of {?mgfp-5?} (B09ig239)
CGTTAGGATCCAGAAGAACCACCACCGTAGTATTT
Gene Synthesis of B09ig249 {<Tev N>}
Pool O09ig249 through O09ig260, assemble by PCA
PCR O09ig249/O09ig260 on PCA reaction (386, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig249 {?Tev N?}
----
O09ig249 PCA assembly of {?Tev N?} (B09ig249)
CCATAGAATTCATGAGATCTGGTGAATCTCTGTTCAAAGGTCCG
O09ig250 PCA assembly of {?Tev N?} (B09ig249)
CAGATGGTAGAAGAGATCGGGTTGTAGTCACGCGGACCTTTGAACAGAGATT
O09ig251 PCA assembly of {?Tev N?} (B09ig249)
CCGATCTCTTCTACCATCTGCCACCTGACCAACGAATCTGACGGTCACACCA
O09ig252 PCA assembly of {?Tev N?} (B09ig249)
GAACGGACCGAAACCGATACCGTACAGAGAGGTGGTGTGACCGTCAGATTCG
O09ig253 PCA assembly of {?Tev N?} (B09ig249)
GTATCGGTTTCGGTCCGTTCATCATCACCAACAAACACCTGTTCCGTCGTAA
O09ig254 PCA assembly of {?Tev N?} (B09ig249)
CGTGGAGGGATTGAACCAGCAGAGTGCCGTTGTTACGACGGAACAGGTGTTT
O09ig255 PCA assembly of {?Tev N?} (B09ig249)
GCTGGTTCAATCCCTCCACGGTGTTTTCAAAGTTAAAAACACCACCACCCTC
O09ig256 PCA assembly of {?Tev N?} (B09ig249)
ATCATGTCACGACCGTCGATGAGATGTTGTTGGAGGGTGGTGGTGTTTTTAA
O09ig257 PCA assembly of {?Tev N?} (B09ig249)
ATCGACGGTCGTGACATGATTATTATTCGTATGCCAAAGGATTTTCCGCCGT
O09ig258 PCA assembly of {?Tev N?} (B09ig249)
CTGCGGTTCACGGAATTTGAGCTTTTGCGGGAACGGCGGAAAATCCTTTGGC
O09ig259 PCA assembly of {?Tev N?} (B09ig249)
TCAAATTCCGTGAACCGCAGCGTGAAGAACGTATCTGCCTCGTAACTACCAA
O09ig260 PCA assembly of {?Tev N?} (B09ig249)
CGTTAGGATCCGGTCTGGAAGTTGGTAGTTACGAGGCAGAT
Gene Synthesis of B09ig261 {<Tev C>}
Pool O09ig261 through O09ig276, assemble by PCA
PCR O09ig261/O09ig276 on PCA reaction (403, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig261 {?Tev C?}
----
O09ig261 PCA assembly of {?Tev C?} (B09ig261)
CCATAGAATTCATGAGATCTAAATCTATGTCCTCTATG
O09ig262 PCA assembly of {?Tev C?} (B09ig261)
GGAAGGTGCAAGAGGTGTCAGAAACCATAGAGGACATAGATTTAG
O09ig263 PCA assembly of {?Tev C?} (B09ig261)
TGACACCTCTTGCACCTTCCCATCCTCTGACGGTATCTTCTGGAA
O09ig264 PCA assembly of {?Tev C?} (B09ig261)
ACCGTCTTTGGTCTGAATCCAGTGTTTCCAGAAGATACCGTCAGA
O09ig265 PCA assembly of {?Tev C?} (B09ig261)
GGATTCAGACCAAAGACGGTCAGTGCGGTTCTCCGCTGGTTTCTA
O09ig266 PCA assembly of {?Tev C?} (B09ig261)
ATACCAACGATGAAACCGTCACGGGTAGAAACCAGCGGAGAACCG
O09ig267 PCA assembly of {?Tev C?} (B09ig261)
GACGGTTTCATCGTTGGTATCCACTCTGCTTCTAACTTCACCAAC
O09ig268 PCA assembly of {?Tev C?} (B09ig261)
GAACAGAGGTGAAGTAGTTGTTGGTGTTGGTGAAGTTAGAAGCAG
O09ig269 PCA assembly of {?Tev C?} (B09ig261)
CAACTACTTCACCTCTGTTCCAAAGAATTTTATGGAACTGCTGAC
O09ig270 PCA assembly of {?Tev C?} (B09ig261)
AACCCATTGTTGTGCTTCCTGGTTGGTCAGCAGTTCCATAAAATT
O09ig271 PCA assembly of {?Tev C?} (B09ig261)
AGGAAGCACAACAATGGGTTTCTGGTTGGCGTCTGAACGCTGACT
O09ig272 PCA assembly of {?Tev C?} (B09ig261)
ACTTTGTGACCACCCCACAGAACAGAGTCAGCGTTCAGACGCCAA
O09ig273 PCA assembly of {?Tev C?} (B09ig261)
CTGTGGGGTGGTCACAAAGTTTTCATGTCTAAACCGGAAGAACCG
O09ig274 PCA assembly of {?Tev C?} (B09ig261)
GGGTAGCTTCTTTAACCGGCTGGAACGGTTCTTCCGGTTTAGACA
O09ig275 PCA assembly of {?Tev C?} (B09ig261)
GCCGGTTAAAGAAGCTACCCAACTCATGTCTGAACTGGTTTACTC
O09ig276 PCA assembly of {?Tev C?} (B09ig261)
CGTTAGGATCCCTGAGAGTAAACCAGTTCAGACAT
PCR Construction of {<espP(beta)>}
PCR ca998/O09ig280 on M10044 (1362, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09espP {<espP(beta)>}
----
ca998 Forward Oligo
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig280 Reverse Oligo for the removal of the stop codon from {<espP(beta)>}
CGTTAGGATCCgaacgagtaacggaaattagcg
PCR Construction of {<upaG_short>}
PCR ca998/O09ig277 on M10026 (279, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09upaG {<espP(beta)>}
----
ca998 Forward Oligo
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig277 Reverse Oligo for the removal of stop codon from {<upaG_short>}
CGTTAGGATCCccactgaataccggcaccgagtgcg
PCR Construction of {<CPG_L2>}
PCR ca998/O09ig278 on M10086 (873, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09cpgl2 {<CPG_L2>}
----
ca998 Forward Oligo
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig278 Reverse Oligo for the removal of stop codon from {<CPG_L2>}
CGTTAGGATCCcgcgctataatctaccggcccttcc
PCR Construction of {<CPG_L6>}
PCR ca998/O09ig279 on M10011 (873, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09cpgl6 {<CPG_L6>}
----
ca998 Forward Oligo
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09cpgl6 Reverse Oligo for the removal of stop codon from {<CPG_L6>}
CGTTAGGATCCctgccagtcccagttactccagcg
PCR Construction of {<TshA>}
PCR ca998/O09ig283 on M10088 (1575, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-O09ig283 {<TshA>}
-------------------------------------
Ca998 forward oligo
gtatcacgaggcagaatttcag
O09ig283 reverse oligo with stop codons removed for {<TshA>}
gacggGGATCCgaatgaataacgaatattag
PCR Construction of {<eCPX>}
PCR ca998/O09ig282 on M10014 (477, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-O09ig282 {<eCPX>}
-------------------------------------
Ca998 forward oligo
gtatcacgaggcagaatttcag
O09ig282 reverse oligo with stop codons removed for {<eCPX>}
ccggtGGATCCAGAGCTTGCAGTACGGCTTT
PCR Construction of {<ehaB>}
PCR ca998/O09ig281 on M10052 (1229, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-O09ig281 {<ehaB>}
-------------------------------------
Ca998 forward oligo
gtatcacgaggcagaatttcag
O09ig281 reverse oligo with stop codons removed for {<ehaB>}
cggtaGGATCCccaggtatatttaacaccca
SOEing construction of B09ig302 {<VirG AtD>}
PCR O09ig302/O09ig301 on S. Flexneri (490bp, gp = frag1)
PCR O09ig300/O09ig303 on S. Flexneri (986bp, gp = frag2)
----
PCR O09ig302/O09ig303 on mixed frags (1457bp, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig302 {?VirG AtD?}
----
O09ig302 Forward PCR of {?VirG AtD?} (B09ig302)
AAGTGGAATTCATGAGATCTTCGTCAAATGTCGGTCAGAC
O09ig301 Reverse internal oligo for {?VirG AtD?} (B09ig302)
CTGCCAGCCTCAGGGCGCC
O09ig300 Forward internal oligo for {?VirG AtD?} (B09ig302)
GGCGCCCTGAGGCTGGCAG
O09ig303 Reverse PCR of {?VirG AtD?} (B09ig302)
GTTAGGGATCCGAAGGTATATTTCACACCC
SOEing construction of B09ig306 {<yuaQ AtD>}
PCR O09ig306/O09ig305 on MG1655(ask chris about EcoRV before start) (464bp, gp = frag1)
PCR O09ig304/O09ig307 on MG1655(ask chris about EcoRV before start) (452bp, gp = frag2)
----
PCR O09ig306/O09ig307 on mixed frags (898bp, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig306 {?yuaQ AtD?}
----
O09ig306 Forward PCR of {?yuaQ AtD?} (B09ig306)
AAGTGGAATTCATGAGATCTGGAGAACCGAAAGCAACCAG
O09ig305 Reverse internal oligo for {?yuaQ AtD?} (B09ig306)
GCTGCTGAACTCCCCGATTTTC
O09ig304 Forward internal oligo for {?yuaQ AtD?} (B09ig306)
GAAAATCGGGGAGTTCAGCAGC
O09ig307 Reverse PCR of {?yuaQ AtD?} (B09ig306)
GTTAGGGATCCGAATGTATACTTCACACCCAG
SOEing construction of B09ig310 {<AIDA-1 AtD>}
PCR O09ig310/O09ig309 on EHEC (1184bp, gp = frag1)
PCR O09ig308/O09ig311 on EHEC (287bp, gp = frag2)
----
PCR O09ig310/O09ig311 on mixed frags (1450bp, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig310 {?AIDA-1 AtD?}
----
O09ig310 Forward PCR of {?AIDA-1 AtD?} (B09ig310)
AAGTGGAATTCATGAGATCTGCTGGCAATGTGTTAGTGGTG
O09ig309 Reverse internal oligo for {?AIDA-1 AtD?} (B09ig310)
ATGGTGGCTCTTGAGCCAGGTTTT
O09ig308 Forward internal oligo for {?AIDA-1 AtD?} (B09ig310)
AAAACCTGGCTCAAGAGCCACCAT
O09ig311 Reverse PCR of {?AIDA-1 AtD?} (B09ig310)
GTTAGGGATCCGAATTGCCACTTAATGCCAAC
SOEing construction of B09ig314 {<cel3A!}
PCR O09ig314/O09ig313 on TEMPLATE (2413bp, gp = frag1)
PCR O09ig312/O09ig315 on TEMPLATE (171bp, gp = frag2)
----
PCR O09ig314/O09ig315 on mixed frags (2566bp, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig314 {?cel3A!}
----
O09ig314 Forward PCR of {?cel3A!} (B09ig314)
AAGTGGAATTCATGAGATCTGGTTGTGATTCCCGCGCTCC
O09ig313 Reverse internal oligo for {?cel3A!} (B09ig314)
GCGCCGGAGTTCGGGTAG
O09ig312 Forward internal oligo for {?cel3A!} (B09ig314)
CTACCCGAACTCCGGCGC
O09ig315 Reverse PCR of {?cel3A!} (B09ig314)
GTTAGGGATCCGGGGCAGGCGACGTCTTTTC
SOEing construction of B09ig318 {<cel5B!}
PCR O09ig318/O09ig317 on TEMPLATE (1226bp, gp = frag1)
PCR O09ig316/O09ig319 on TEMPLATE (418bp, gp = frag2)
----
PCR O09ig318/O09ig319 on mixed frags (1625bp, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig318 {?cel5B!}
----
O09ig318 Forward PCR of {?cel5B!} (B09ig318)
AAGTGGAATTCATGAGATCTGATGTCGCGCCGCTGTCCGTAC
O09ig317 Reverse internal oligo for {?cel5B!} (B09ig318)
CAGGCTGGACGAGCTGGAG
O09ig316 Forward internal oligo for {?cel5B!} (B09ig318)
CTCCAGCTCGTCCAGCCTG
O09ig319 Reverse PCR of {?cel5B!} (B09ig318)
GTTAGGGATCCATTGTTACAAACATTACCGC
SOEing construction of B09ig322 {<cel9A!}
PCR O09ig322/O09ig321 on TEMPLATE (1055bp, gp = frag1)
PCR O09ig320/O09ig323 on TEMPLATE (1784bp, gp = frag2)
----
PCR O09ig322/O09ig323 on mixed frags (2821bp, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig322 {?cel9A!}
----
O09ig322 Forward PCR of {?cel9A!} (B09ig322)
AAGTGGAATTCATGAGATCTGAGGTGGGTAACCCCCGTGTC
O09ig321 Reverse internal oligo for {?cel9A!} (B09ig322)
CCAATAGAACTCATCGGC
O09ig320 Forward internal oligo for {?cel9A!} (B09ig322)
GCCGATGAGTTCTATTGG
O09ig323 Reverse PCR of {?cel9A!} (B09ig322)
GTTAGGGATCCCTGACATACACTTCCGGTTAC
Basic PCR construction of B09ig324 {<SOD>}
PCR O09ig324/O09ig325 on MG1655 (ask chris abuot EcoRV before start) (493, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig324 {?SOD?}
----
O09ig324 Forward PCR of {?SOD?} (B09ig324)
CCATAGAATTCATGAGATCTGCCAGTGAAAAAGTCGAGATG
O09ig325 Reverse PCR of {?SOD?} (B09ig324)
CGTTAGGATCCCTTAATTACACCACAGGCATAG
Basic PCR construction of B09ig326 {<Tir-M>}
PCR O09ig326/O09ig327 on E2348/69(O127:H6)EPEC (352, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig326 {?Tir-M?}
----
O09ig326 Forward PCR of {?Tir-M?} (B09ig326)
CCATAGAATTCATGAGATCTCAGGCGTTGGCTTTGACACC
O09ig327 Reverse PCR of {?Tir-M?} (B09ig326)
CGTTAGGATCCTGAAAGCTGTAATTCCTCCTG
Basic PCR construction of B09ig328 {<cel6A!}
PCR O09ig328/O09ig329 on TEMPLATE (2041, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig328 {?cel6A!}
----
O09ig328 Forward PCR of {?cel6A!} (B09ig328)
CCATAGAATTCATGAGATCTGCATGTACCTACAAAATTAC
O09ig329 Reverse PCR of {?cel6A!} (B09ig328)
CGTTAGGATCCTTGCAGTGGTGGATAAGCG
