BYU iGEM/Notebook/5 January 2011

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5 January 2011

Colon Cancer Detection Questions

Can isopeptags be used to detect a cancer biomarker and then create a response inside the cell? If not, what other transmembrane proteins can?

Heat Production

Diagram of proposed mechanism

What's a good "cold switch" - a riboswitch that would stop transcription at a specific temperature?

Does the UCP1 create enough heat, fast enough, to make a difference?

Does activation of UCP1 harmfully inhibit ATP production? Or can we overproduce ATP to compensate?

CO Detection

Can E. coli be altered to survive in CO long enough to detect it?

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