BME100 s2018:Group3 W1030 L4
|BME 100 Spring 2018|| Home |
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Wiki Editing Help
LAB 4 WRITE-UP
Research and Development
PCR - The Underlying Technology
The PCR reaction has multiple components in order to make the reaction work. The components are Template DNA, Primers, Taq Polymerase, and Deoxyribonucleotides. The template DNA is a DNA sample that is used to target the specific DNA copies. The primers attach to the end of the DNA strand once the strands has been separated. Then they would copy the specific targeted DNA sequence. The taq polymerase reads the DNA sequence and translate it to match the matching the Nucleotides thus making a additional DNA copy. The Nucleotides are the codes that make up DNA strand.
1. Initial Step (95°C for 2 minutes): The template DNA strands comes to temperature.
2. Denature (95°C for 30 seconds): The template DNA separates into two individual strands.
3. Anneal (57°C for 30 seconds): The primers attaches at the end of each DNA strand.
4. Extend (72°C for 30 seconds): The taq polymerase is activated and attached to the primers.
5. Final Step (72°C for 2 minutes): The polymerase begins to add complementary nucleotides on DNA stands. Which replicates the targeted DNA.
6. Final Hold (4°C): Copies are made.
The main cycle that base pairing occurs is in the Anneal and Extend Cycle because that is when the taq polymerase is activated and attached to the primers. Once the primers and taq polymerase are attached, they begin to add complementary nucleotides to make a copy of the template DNA.
SNP Information & Primer Design
Background: About the Disease SNP The species that this variation is found in is Homo Sapiens, and it is located on the 6:131851228 chromosome. This variation has been linked to metabolic diseases. ENPP1 is the ectonucleotide pyrophosphate/ phosphodiesterase, and its function is for zinc ion binding, insulin receptor binding, and protein binding. The disease-associated codon is CAG. It is located at 131851228.