BISC220/S12: Mod 1 Media
From OpenWetWare
Jump to navigationJump to search
β-galactosidase Series Media Recipes
Solution | Composition |
---|---|
Luria Broth | 1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar (only in plates) |
Ampicillin | Stock = 25 mg/ml in water |
IPTG (Isopropyl β-D-1-thiogalactopyranoside) | Stock 1 mg/ml |
Na2CO3 | Stock 1M |
Z-buffer | 60 mM Na2HPO4 60 mM NaH2PO4 1 mM MgSO4 10 mM HCl 0.27% β-mercaptoethanol |
ONPG (ortho-Nitrophenyl-β-galactoside) | Stock 4 mg/ml |
Laemmli Solution (2x Sample Buffer) | 0.125 M Tris, pH 6.7 4% SDS 20% glycerol 10% β-mercaptoethanol |
Gel Running Buffer | 0.92 M Glycine 0.05 M Tris Base 0.1% SDS |
GelCode Blue Stain Reagent | Thermo Scientific |