User:Allison K. Alix/Notebook/Thesis Research/2013/04/05

From OpenWetWare
< User:Allison K. Alix‎ | Notebook‎ | Thesis Research‎ | 2013‎ | 04
Revision as of 06:00, 5 April 2013 by Allison K. Alix (talk | contribs) (Procedures)
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


  • Mix DNA with higher concentration of ThT
  • Measure absorbance and fluorescence of DNA/ThT hybrid


Part 1: Mixing thiol-DNA with ThT

1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA

  • Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA

2) Place on heat block at ~76°C for 25 minutes

3) Allow solution to cool on heat block until at room temperature

4) Take absorbance and fluorescence measurements

  • AuNP will then be added to this solution