Objectives
- Measure absorbance and fluorescence of thiol-DNA/MB and thiol-DNA/ThT mixtures prepared on 04/02/2013
Procedures
Part 1: Measure absorbance values of hybrid
thiol-DNA/ThT
fluorescence:
1) excitation at 400. Start 410, end 610 (according to absorbance spec
2) excitation at 440 start at 450 end 650
3) excitation at 417 start at 427 end 650
Part 2: Measure fluorescence of ThT and DNA alone
Observations
- The concentration of ThT used in this solution was likely too low. Although ThT was in excess, the absorbance seen on the UV-Vis spectrum was very low. It is interesting to note that the DNA-ThT hybrid did show slight fluorescence intensity. Because there is a possibility that this signal was a background signal coming from the ThT, a fluorescence spectrum of that same concentration of ThT alone in solution was also taken (See Data below)
Data
Absorbance:
Fluorescence:
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