Difference between revisions of "Sauer:Protocols"

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(More ''in vivo'' goodies)
(Protein Expression/Purification)
 
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[[Sauer:ClpP purification]]  
 
[[Sauer:ClpP purification]]  
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 +
[[Sauer:ClpA purification]]
  
 
[[Sauer:SspB purification]]
 
[[Sauer:SspB purification]]
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[[Sauer:Lysing E. coli with Lysozymes]]
 
[[Sauer:Lysing E. coli with Lysozymes]]
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[[Sauer:GFP-H6-ssrA purification]]
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 +
[[Sauer:GST-sFtsH purification]]
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[[Sauer:FtsH purification]]
  
 
===''In vitro'' enzymatic assays===
 
===''In vitro'' enzymatic assays===
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[[Sauer:ATPase assays]]
 
[[Sauer:ATPase assays]]
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[[Sauer:In vitro peptide degradation by ClpXP]]
  
 
===Proteins: ''in vitro''===
 
===Proteins: ''in vitro''===
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[[Sauer:P1vir phage transduction]]
 
[[Sauer:P1vir phage transduction]]
  
[[Sauer:Plasmid-born lambdaRed recombineering using dsDNA]]
+
[[Sauer:Plasmid-born λRed recombineering using dsDNA]]
  
 
===Other good stuff===
 
===Other good stuff===
 
[[Library Generation|Generation of genomic libraries]]
 
[[Library Generation|Generation of genomic libraries]]
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[[Sauer:HPLC protocol]]
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[[Sauer:FPLC protocol]]
  
 
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</div>

Latest revision as of 13:34, 27 July 2009

SAUER LAB

Home        Protocols        Lab Members        Materials        Equipment        Links        Internal       

Please link protocols to the general Protocols page if appropriate.

Protein Expression/Purification

Sauer:ClpX purification

Sauer:His6 ClpX purification

Sauer:ClpP purification

Sauer:ClpA purification

Sauer:SspB purification

Sauer:Expression/purification of 35S-Met proteins

Sauer:Purification of His6-tagged proteins

Sauer:Lysing E. coli with Lysozymes

Sauer:GFP-H6-ssrA purification

Sauer:GST-sFtsH purification

Sauer:FtsH purification

In vitro enzymatic assays

Sauer:In vitro degradation of GFP-ssrA by ClpXP

Sauer:In vitro degradation of GFP-ssrA by ClpAP

Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED!

Sauer:ATPase assays

Sauer:In vitro peptide degradation by ClpXP

Proteins: in vitro

Sauer:Silver staining

Sauer:bis-Tris SDS-PAGE, the very best

Nucleic acids: in vitro

Sauer:In vitro transcription with T7 RNA polymerase

Sauer:Phenol/chloroform extraction

Quantitating nucleic acids

Sauer:Site-directed mutagenesis

Sauer:RNA Purification from E. coli

Sauer:Stitching Genes by PCR