User contributions
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- 09:37, 26 February 2010 (diff | hist) . . (+16) . . Sauer:StockRoomTable (current)
- 09:36, 26 February 2010 (diff | hist) . . (-7) . . Sauer:StockRoomTable
- 14:28, 19 August 2009 (diff | hist) . . (+1,433) . . Jhdavis::MetCleavage (current)
- 10:34, 19 August 2009 (diff | hist) . . (0) . . N File:FPLCsignupSauer.xls (current)
- 10:34, 19 August 2009 (diff | hist) . . (+34) . . Sauer:FPLC (current)
- 12:35, 23 July 2009 (diff | hist) . . (0) . . File:SauerLabJobList.xls (uploaded a new version of "Image:SauerLabJobList.xls") (current)
- 12:32, 23 July 2009 (diff | hist) . . (0) . . N File:SauerLabJobList.xls
- 12:31, 23 July 2009 (diff | hist) . . (+29) . . Sauer:Lab jobs (current)
- 12:29, 23 July 2009 (diff | hist) . . (-1,894) . . Sauer:Lab jobs (Removing all content from page)
- 11:50, 18 May 2009 (diff | hist) . . (+8) . . Sauer:Making yeg plates (current)
- 11:27, 6 May 2009 (diff | hist) . . (+317) . . N Jhdavis::4x reducing SDS-PAGE loading buffer (New page: 4X SDS-PAGE sample loading buffer From OpenWetWare 10 ml 1X 2.0 ml 1M Tris-HCl, pH 6.8 50 mM 0.8 g SDS 2% 4.0 ml 100% glycerol 10% 0.4 ml 14.7 M -mercaptoethanol 1% 1.0 ...) (current)
- 11:26, 6 May 2009 (diff | hist) . . (+50) . . User:Jhdavis (→Buffers) (current)
- 13:55, 1 May 2009 (diff | hist) . . (+362) . . N Sauer:Davis Minimal Media (New page: Davis Minimal Media (10x) 1 L: K2HPO4 - 90 gr KH2PO4 - 30 gr (NH4)SO4 - 20 gr NaCitrate - 5 gr MgSO4100x (100gr/L) (1%) Glucose100x 20% vitamin B1 (thiamine)2000x 2 mg/mL Amino Ac...) (current)
- 13:49, 1 May 2009 (diff | hist) . . (+31) . . User:Jhdavis
- 13:30, 30 April 2009 (diff | hist) . . (+11) . . Jhdavis::Immunoprecip (current)
- 13:29, 30 April 2009 (diff | hist) . . (+453) . . N Jhdavis::Immunoprecip (New page: Immunoprecipitation (sherman et al. 1992 EMBO J 11: 71-77) 20 mL culture, mid-log phase centrifuge 5 min, 5000 rmp resuspend in 2 mL of 10 mM tris pH 8.0, 1 mM EDTA, 1mg/mL lysozyme freez...)
- 13:27, 30 April 2009 (diff | hist) . . (+87) . . User:Jhdavis (→Protocols)
- 12:38, 29 March 2009 (diff | hist) . . (+37) . . Sauer:StockRoomTable
- 12:36, 29 March 2009 (diff | hist) . . (+38) . . Sauer:group meeting food schedule (current)
- 12:35, 29 March 2009 (diff | hist) . . (+6) . . Sauer:group meeting food schedule
- 12:35, 29 March 2009 (diff | hist) . . (-7) . . Sauer:group meeting food schedule
- 12:35, 29 March 2009 (diff | hist) . . (+14) . . Sauer:group meeting food schedule
- 12:30, 29 March 2009 (diff | hist) . . (-31) . . Sauer:group meeting food schedule
- 08:08, 16 March 2009 (diff | hist) . . (-1) . . Sauer:Stock room items
- 10:53, 13 March 2009 (diff | hist) . . (+20) . . Sauer:In vitro degradation of GFP-ssrA by ClpXP (→ATP regeneration mix (1X)) (current)
- 09:14, 13 March 2009 (diff | hist) . . (+83) . . Sauer:Stock room items (/* We currently have a bunch of extra tips on the wall opposing the stock shelves, check these before you buy more (art P1000 in particular). Also, a bunch of vendors have given us free filter tips,)
- 09:14, 13 March 2009 (diff | hist) . . (+98) . . Sauer:Stock room items (→There are a ton of extra gloves (diamond-grip in particular), check the boxes below the glove shelf before buying more.)
- 09:02, 13 March 2009 (diff | hist) . . (-1) . . Sauer:Stock room items
- 09:02, 13 March 2009 (diff | hist) . . (+636) . . Sauer:Stock room items
- 09:32, 9 March 2009 (diff | hist) . . (0) . . N File:FplcSingup.xlsx (current)
- 09:32, 9 March 2009 (diff | hist) . . (+25) . . N Jhdavis::fplc singup (New page: Image:fplcSingup.xlsx) (current)
- 09:31, 9 March 2009 (diff | hist) . . (+26) . . User:Jhdavis
- 14:40, 4 March 2009 (diff | hist) . . (+8) . . Jhdavis::atpaseNotes (current)
- 13:04, 8 February 2009 (diff | hist) . . (-24) . . Jhdaivs::SumoPrep (current)
- 13:03, 8 February 2009 (diff | hist) . . (+23) . . Jhdaivs::SumoPrep
- 12:20, 8 February 2009 (diff | hist) . . (+47) . . Jhdaivs::SumoPrep
- 17:30, 4 February 2009 (diff | hist) . . (+172) . . N Jhdavis::atpaseNotes (New page: 50 uL rxns - read at 340 nm 25 uL sample, 25 uL 2x nadh atpase system (made from 20x stock in PD buffer) totalRate = slope/pathlength/6.23 for floppy plates, pathlength =)
- 17:19, 4 February 2009 (diff | hist) . . (+39) . . User:Jhdavis
- 16:43, 4 February 2009 (diff | hist) . . (0) . . Sauer:ATPase assays (current)
- 07:15, 4 February 2009 (diff | hist) . . (+816) . . N Jhdavis::regenQiagen (New page: We use Qiagen columns and regenerate them using the HCl method as published (http://www.hiv-vkrc.org/regenration.php) with the exception that we incubate in 1 N HCl for no longer than 12 h...) (current)
- 07:14, 4 February 2009 (diff | hist) . . (+54) . . User:Jhdavis
- 14:52, 3 February 2009 (diff | hist) . . (+59) . . Jhdaivs::SumoPrep
- 14:36, 3 February 2009 (diff | hist) . . (+571) . . N Jhdaivs::SumoPrep (New page: ulp1 has c-term his tag for purification expressed from pET24d in rosetta - (DE3) pLysS - grown in lb/2xyt/cam/kan - 1 L - induced 1 mM iptg 4 hours resuspended in 20 mM tris pH 8.0, 15...)
- 14:29, 3 February 2009 (diff | hist) . . (+80) . . User:Jhdavis
- 13:08, 26 January 2009 (diff | hist) . . (+18) . . Jhdavis::ClpPHisPrep (current)
- 13:07, 26 January 2009 (diff | hist) . . (-4) . . Jhdavis::ClpPHisPrep
- 13:07, 26 January 2009 (diff | hist) . . (+1,764) . . N Jhdavis::ClpPHisPrep (New page: E. coli ClpP protein with a C-terminal His6 tag was expressed in E. coli strain DH5α/pYK133/pRep4. Cultures were grown at 30°C to an OD600 of 0.5 in LB with antibiotics, and IPTG was a...)
- 13:03, 26 January 2009 (diff | hist) . . (+54) . . User:Jhdavis (→Protocols)
- 08:35, 4 November 2008 (diff | hist) . . (+1,606) . . N User:Jhdavis/Notebook/SauerLab2008 (Autocreated Lab Notebook name=User:Jhdavis/Notebook/SauerLab2008, content from MediaWiki:ProjectContentDefault)
- 08:35, 4 November 2008 (diff | hist) . . (+945) . . N User:Jhdavis/Notebook/SauerLab2008/Entry Base (Autocreated Lab Notebook name=User:Jhdavis/Notebook/SauerLab2008/Entry Base, content from MediaWiki:EntryContentDefault)
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