User contributions for Jhdavis

26 February 2010

• 16:3716:37, 26 February 2010 diff hist +16 Sauer:StockRoomTable No edit summary current
• 16:3616:36, 26 February 2010 diff hist −7 Sauer:StockRoomTable No edit summary

19 August 2009

• 21:2821:28, 19 August 2009 diff hist +1,433 Jhdavis::MetCleavage No edit summary current
• 17:3417:34, 19 August 2009 diff hist 0 N File:FPLCsignupSauer.xls No edit summary current
• 17:3417:34, 19 August 2009 diff hist +34 Sauer:FPLC No edit summary current

23 July 2009

• 19:3519:35, 23 July 2009 diff hist 0 File:SauerLabJobList.xls uploaded a new version of "Image:SauerLabJobList.xls" current
• 19:3219:32, 23 July 2009 diff hist 0 N File:SauerLabJobList.xls No edit summary
• 19:3119:31, 23 July 2009 diff hist +29 Sauer:Lab jobs No edit summary current
• 19:2919:29, 23 July 2009 diff hist −1,894 Sauer:Lab jobs Removing all content from page

18 May 2009

• 18:5018:50, 18 May 2009 diff hist +8 Sauer:Making yeg plates No edit summary current

6 May 2009

• 18:2718:27, 6 May 2009 diff hist +317 N Jhdavis::4x reducing SDS-PAGE loading buffer New page: 4X SDS-PAGE sample loading buffer From OpenWetWare 10 ml 1X 2.0 ml 1M Tris-HCl, pH 6.8 50 mM 0.8 g SDS 2% 4.0 ml 100% glycerol 10% 0.4 ml 14.7 M -mercaptoethanol 1% 1.0 ... current
• 18:2618:26, 6 May 2009 diff hist +50 User:Jhdavis →Buffers current

1 May 2009

• 20:5520:55, 1 May 2009 diff hist +362 N Sauer:Davis Minimal Media New page: Davis Minimal Media (10x) 1 L: K2HPO4 - 90 gr KH2PO4 - 30 gr (NH4)SO4 - 20 gr NaCitrate - 5 gr MgSO4100x (100gr/L) (1%) Glucose100x 20% vitamin B1 (thiamine)2000x 2 mg/mL Amino Ac... current
• 20:4920:49, 1 May 2009 diff hist +31 User:Jhdavis No edit summary

30 April 2009

• 20:3020:30, 30 April 2009 diff hist +11 Jhdavis::Immunoprecip No edit summary current
• 20:2920:29, 30 April 2009 diff hist +453 N Jhdavis::Immunoprecip New page: Immunoprecipitation (sherman et al. 1992 EMBO J 11: 71-77) 20 mL culture, mid-log phase centrifuge 5 min, 5000 rmp resuspend in 2 mL of 10 mM tris pH 8.0, 1 mM EDTA, 1mg/mL lysozyme freez...
• 20:2720:27, 30 April 2009 diff hist +87 User:Jhdavis →Protocols

29 March 2009

• 19:3819:38, 29 March 2009 diff hist +37 Sauer:StockRoomTable No edit summary
• 19:3619:36, 29 March 2009 diff hist +38 Sauer:group meeting food schedule No edit summary current
• 19:3519:35, 29 March 2009 diff hist +6 Sauer:group meeting food schedule No edit summary
• 19:3519:35, 29 March 2009 diff hist −7 Sauer:group meeting food schedule No edit summary
• 19:3519:35, 29 March 2009 diff hist +14 Sauer:group meeting food schedule No edit summary
• 19:3019:30, 29 March 2009 diff hist −31 Sauer:group meeting food schedule No edit summary

16 March 2009

• 15:0815:08, 16 March 2009 diff hist −1 Sauer:Stock room items No edit summary

13 March 2009

• 17:5317:53, 13 March 2009 diff hist +20 Sauer:In vitro degradation of GFP-ssrA by ClpXP →ATP regeneration mix (1X) current
• 16:1416:14, 13 March 2009 diff hist +83 Sauer:Stock room items /* We currently have a bunch of extra tips on the wall opposing the stock shelves, check these before you buy more (art P1000 in particular). Also, a bunch of vendors have given us free filter tips,
• 16:1416:14, 13 March 2009 diff hist +98 Sauer:Stock room items →There are a ton of extra gloves (diamond-grip in particular), check the boxes below the glove shelf before buying more.
• 16:0216:02, 13 March 2009 diff hist −1 Sauer:Stock room items No edit summary
• 16:0216:02, 13 March 2009 diff hist +636 Sauer:Stock room items No edit summary

9 March 2009

• 16:3216:32, 9 March 2009 diff hist 0 N File:FplcSingup.xlsx No edit summary current
• 16:3216:32, 9 March 2009 diff hist +25 N Jhdavis::fplc singup New page: Image:fplcSingup.xlsx current
• 16:3116:31, 9 March 2009 diff hist +26 User:Jhdavis No edit summary

4 March 2009

• 21:4021:40, 4 March 2009 diff hist +8 Jhdavis::atpaseNotes No edit summary current

8 February 2009

• 20:0420:04, 8 February 2009 diff hist −24 Jhdaivs::SumoPrep No edit summary current
• 20:0320:03, 8 February 2009 diff hist +23 Jhdaivs::SumoPrep No edit summary
• 19:2019:20, 8 February 2009 diff hist +47 Jhdaivs::SumoPrep No edit summary

5 February 2009

• 00:3000:30, 5 February 2009 diff hist +172 N Jhdavis::atpaseNotes New page: 50 uL rxns - read at 340 nm 25 uL sample, 25 uL 2x nadh atpase system (made from 20x stock in PD buffer) totalRate = slope/pathlength/6.23 for floppy plates, pathlength =
• 00:1900:19, 5 February 2009 diff hist +39 User:Jhdavis No edit summary

4 February 2009

• 23:4323:43, 4 February 2009 diff hist 0 Sauer:ATPase assays No edit summary current
• 14:1514:15, 4 February 2009 diff hist +816 N Jhdavis::regenQiagen New page: We use Qiagen columns and regenerate them using the HCl method as published (http://www.hiv-vkrc.org/regenration.php) with the exception that we incubate in 1 N HCl for no longer than 12 h... current
• 14:1414:14, 4 February 2009 diff hist +54 User:Jhdavis No edit summary

3 February 2009

• 21:5221:52, 3 February 2009 diff hist +59 Jhdaivs::SumoPrep No edit summary
• 21:3621:36, 3 February 2009 diff hist +571 N Jhdaivs::SumoPrep New page: ulp1 has c-term his tag for purification expressed from pET24d in rosetta - (DE3) pLysS - grown in lb/2xyt/cam/kan - 1 L - induced 1 mM iptg 4 hours resuspended in 20 mM tris pH 8.0, 15...
• 21:2921:29, 3 February 2009 diff hist +80 User:Jhdavis No edit summary

26 January 2009

• 20:0820:08, 26 January 2009 diff hist +18 Jhdavis::ClpPHisPrep No edit summary current
• 20:0720:07, 26 January 2009 diff hist −4 Jhdavis::ClpPHisPrep No edit summary
• 20:0720:07, 26 January 2009 diff hist +1,764 N Jhdavis::ClpPHisPrep New page: E. coli ClpP protein with a C-terminal His6 tag was expressed in E. coli strain DH5α/pYK133/pRep4. Cultures were grown at 30°C to an OD600 of 0.5 in LB with antibiotics, and IPTG was a...
• 20:0320:03, 26 January 2009 diff hist +54 User:Jhdavis →Protocols

4 November 2008

• 15:3515:35, 4 November 2008 diff hist +945 N User:Jhdavis/Notebook/SauerLab2008/Entry Base Autocreated Lab Notebook name=User:Jhdavis/Notebook/SauerLab2008/Entry Base, content from MediaWiki:EntryContentDefault
• 15:3515:35, 4 November 2008 diff hist +1,606 N User:Jhdavis/Notebook/SauerLab2008 Autocreated Lab Notebook name=User:Jhdavis/Notebook/SauerLab2008, content from MediaWiki:ProjectContentDefault