User:Jhdavis

I recently joined the Sauer Lab. clpX holla!!!!

Protocols

1. Western Blots by Eyal Jhdavis::Western::Eyal
2. GFP Purification by organic extraction Jhdavis::GFPOrganic
3. Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
4. Peptide cleavage Jhdavis::PeptideCleavage
5. Flp recombinase Jhdavis::Flping
6. ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
7. Sumo Protease expression/purification from Malakhov 2004 Jhdavis::SumoPrep
8. regenerating qiagen columns Jhdavis::regenQiagen
9. atpase notes Jhdavis::atpaseNotes
10. Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Jhdavis::Immunoprecip

Buffers

1. PD Buffer
2. ATP Regeneration Mix
3. YEG-Cl
4. Sauer:Davis Minimal Media
5. Jhdavis::4x reducing SDS-PAGE loading buffer

Info

• Jhdavis::GelFiltrationStandards
• Jhdavis::MetCleavage
• Jhdavis::lacIoperators
• Jhdavis::DNA 1 letter codes
• Jhdavis::Useful pymol
• Jhdavis::A260:A280 DNA:Protein ratios
• Jhdavis::fplc singup

Links

1. Promoter standard
2. novagen plasmid compatibility

Sheets

1. File:Asdfsa.xlsx