Project Summary
Undergrads
Amy Kristofferson Conor McClune Tahoura Samad Daniela Christoph Neyer
Graduate Students Tim Hsiau
Postdocs
Insert names here
Advisors Chris Anderson Terry Johnson
Lab calendar
Lab Notebooks
Group Amy Conor Daniela Tahoura Christoph Tim The Details
Oligonucleotides Gel Pictures Sequencing Construction Files -80 Stocks Assembly Trees New Composite Parts
Procedures
Polymerase Reactions Wobble Reaction Cloning by PCR SOEing PCR PCA Gene Synthesis QuikChange Colony PCR
Cleanup Reactions Regular Zymo Cleanup Small-Frag Zymo Cleanup Zymo Gel Purification
Digestions Reactions EcoRI/BamHI Digest of Wobble Products EcoRI/BamHI Digest of PCR Products BglII Digest of EIPCR Products Ligation of EcoRI/BamHI digests Ligation of EIPCR digests Analytical digests (Mapping)
Bacteria Manipulations Transformation by heat-shock Picking of colonies Miniprep purification of DNA Macherey-Nagel Miniprep -80 Glycerol Stocks Making Competent Cells
Other LR Gateway Transfers EcoRI/BamHI Part Transfers What do I do with my sequencing data? Small Scale Competent Cell Transformation ELISA 2ab Assembly
Subgroup Notebooks
Useful Links
Subgroup Strategies, Overview
