Knight:Protocols

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Knight Lab


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DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Restriction Digest

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis

Escherichia coli

Knight:Preparing electrocompetent cells

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  • This page was last edited on 5 May 2006, at 21:24.
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