IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Notes

Biofilm Detection: Notes

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Project Goals

• Calibrate basic construct F2620 in E. coli
• Get basic construct F2620 working in vitro
• Describe and model differences of construct in vivo and in vitro

• Build final construct with β-galactosidase
• Calibrate final construct with β-galactosidase in E. coli
• Get basic construct with β-galactosidase working in vitro
• Model the construct in vivo and in vitro
• Optimize construct for biofilm detection

• Model the possible function of Hrp in the system
• Test Hrp in the system

• Create small amount of biofilm using E. coli

Project Status

Protocols:

• E. coli testing protocols written
• in vitro protocols being refined

Wet Lab:

• Test plasmid under cloning (ready at Week 7)
• Bricks for final constructs awaiting ligation

Modelling:

• AHL concentration on plux is currently being modelled