Smolke:Protocols
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Josh K. Michener
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Note: If possible, please follow the
standard
protocol format.
Contents
1
Protocols
1.1
Cloning
1.2
Yeast
1.3
Miscellaneous
2
Solutions
Protocols
Cloning
Primer Design
Plasmid prep with Epoch columns and homemade buffers
PCR amplifying inserts from plasmids
When to use phenol-chloroform vs. columns
Restriction digests
Gel extraction
Ligation
Transformation
Colony PCR
Screening by digestion
Sequencing with Elim
Making freezer stocks
Gene assembly
Yeast
Protocols
Pouring yeast dropout plates
Yeast plasmid transformation
Yeast Colony PCR
Anti-V5 Western
Library-scale yeast transformations
Yeast chromosomal modifications
Yeast plasmid prep
Observations and Discussion
CSY3
Miscellaneous
Glass Beads
Poster Printing
Solutions
Pfu Buffer
Taq Buffer
10x DO Media
dNTP Mixture
5-Fluoroorotic acid
Antibiotic Stocks
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