Recipe
| Amino Acid |
Stock Concentration
(g/L) |
Volume Added
(mL/L) |
Final Media Concentration
(mg/L) |
Notes
|
| Adenine sulfate
|
2 |
100 |
20 |
|
| Uracil
|
2 |
100 |
20 |
|
| L-Tryptophan
|
10 |
20 |
20 |
|
| L-Histidine hydrochloride
|
10 |
20 |
20 |
|
| L-Arginine hydrochloride
|
10 |
20 |
20 |
|
| L-Methionine
|
10 |
20 |
20 |
|
| L-Tyrosine
|
2 |
150 |
30 |
add ~5mL 5N NaOH
|
| L-Leucine
|
10 |
60 |
60 |
|
| L-Isoleucine
|
10 |
30 |
30 |
|
| L-Lysine hydrochloride
|
10 |
30 |
30 |
|
| L-Phenylalanine
|
10 |
50 |
50 |
|
| L-Glutamic acid
|
10 |
100 |
100 |
Stored at RT
|
| L-Aspartic acid
|
10 |
100 |
100 |
Stored at RT
|
| L-Valine
|
30 |
50 |
150 |
|
| L-Threonine
|
40 |
50 |
200 |
|
| L-Serine
|
80 |
50 |
400 |
|
| Nanopure Water
|
|
Remainder |
|
|
Notes
- Leave out whichever amino acid(s) you're dropping out, and replace the volume with water.
- Stocks which are stored at RT are in the cabinet with autoclaved media.
Stephanie
|
