Smolke:Protocols/Transformation

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For each sample 1 tube of electrocompetent cells (keep covered in ice at all time) 1 electroporation cuvette (keep on ice) 1 1.5-ml microfuge tube 1 mL SOC 1 LB plate with appropriate antibiotics Gene Pulser setup Exponential 2000 V voltage 25 uF capacitance 200 ohms resistance 1 mm cuvette If transforming non-purified, non-concentrated ligation product, use 0.5-1.0 ul per sample. Time constant should be at least 4.5, generally between 4.7 and 5.0. If sample sparks or has a low time constant, lowering the volume of ligation product is generally more useful than increasing the volume.