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Summary
10x buffer for PCR with Pfu. Makes 10mL.
Ingredients
- 2 mL 1M Tris-HCl pH 8.8
- 333 μL 3M KCl
- 500 μL 20% Triton X-100
- 1 mL 100x BSA
- 200 μL 1M MgSO4
- 5.967 mL H2O
Protocol
- Mix, vortex.
- Store at -20°C
Notes
- We couldn't find any Triton X-100 in the lab. Our best guess is that NP-40 was (previously) used instead. -Jkm 18:18, 2 August 2006 (EDT)
- Should be supplemented with MgCl2 when used. A working concentration of 2.5mM (a 1/20 dilution of 50mM stock) works for most applications.
Source
Unknown. Ask Kristy.
Kristy
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