Smolke:Protocols/Pfu Buffer

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Summary 10x buffer for PCR with Pfu. Makes 10mL. Ingredients 2 mL 1M Tris-HCl pH 8.8 333 μL 3M KCl 500 μL 20% Triton X-100 1 mL 100x BSA 200 μL 1M MgSO4 5.967 mL H2O Protocol Mix, vortex. Store at -20°C Notes We couldn't find any Triton X-100 in the lab. Our best guess is that NP-40 was (previously) used instead. -Jkm 18:18, 2 August 2006 (EDT) Should be supplemented with MgCl2 when used. A working concentration of 2.5mM (a 1/20 dilution of 50mM stock) works for most applications. Source Unknown. Ask Kristy. Contact Kristy