| MODULE
|
DAY
|
DATE
|
LECTURER
|
TA
|
LAB
|
note
|
|
|
|
Tue Feb 3
|
ANS
|
|
Orientation
|
* Introductory Lecture (PDF)
* Intro Pre-Lab, T/R
|
|
|
| 1
|
1
|
Thur Feb 5
|
AJ
|
NC
|
Day 1: Start-up protein engineering
|
* M1D1 Lecture
* M1D1 Pre-Lab
|
| 1
|
2
|
Tue Feb 10
|
AJ
|
NC
|
Day 2: Site-directed mutagenesis
|
* M1D2 Lecture
* M1D2 Pre-Lab
|
| 1
|
3
|
Thur Feb 12
|
AJ
|
NC
|
Day 3: Bacterial amplification of DNA
|
* M1D3 Lecture
* M1D3 Pre-Lab
|
|
|
|
President's Day Feb 16
|
No lab Feb 17 or Feb 18
|
| 1
|
4
|
Thur Feb 19
|
AJ
|
NC
|
Day 4: Prepare expression system
|
* M1D4 Lecture
* M1D4 Pre-Lab
|
| 1
|
5
|
Tue Feb 24
|
AJ
|
NC
|
Day 5: Induce protein and evaluate DNA
|
* M1D5 Lecture
* M1D5 Pre-Lab
|
| 1
|
6
|
Thur Feb 26
|
AJ
|
NC
|
Day 6: Characterize protein expression
|
* M1D6 Lecture
* M1D6 Pre-Lab
|
| 1
|
7
|
Tue Mar 3
|
AJ
|
NC
|
Day 7: Assay protein behavior
|
* M1D7 Lecture
|
| 1
|
8
|
Thur Mar 5
|
AJ
|
NC
|
Day 8: Data analysis
|
* M1D8 Lecture
|
|
|
| 2
|
1
|
Tue Mar 10
|
LS
|
SG
|
Day 1: siRNA design and introduction to cell culture
|
Module 1 Draft Due
* M2D1 Lecture
* M2D1 Pre-Lab
|
| 2
|
2
|
Thur Mar 12
|
LS
|
SG
|
Day 2: Journal article discussion
|
* M2D2 Lecture
* M2D2 Pre-Lab, part 1
*Atissa's slides on her user page
* M2D2 Pre-Lab, part 2
|
| 2
|
3
|
Tue Mar 17
|
LS
|
SG
|
Day 3: Journal Club
|
NO LECTURE
|
| 2
|
4
|
Thur Mar 19
|
LS
|
SG
|
Day 4: Journal Club
|
Lecture: feedback on Module 1 draft reports
*Neal's slides on his user page
|
|
|
|
Spring Break Mar 23-27
|
No lab all week
|
| 2
|
5
|
Tue Mar 31
|
LS
|
SG
|
Day 5: Transfection
|
* M2D5 Lecture
* M2D5 Pre-Lab
|
| 2
|
6
|
Thur Apr 2
|
LS
|
SG
|
Day 6: Luciferase assays and RNA prep
|
Module 1 Final Report Due
* M2D6 Lecture
* M2D6 Pre-Lab
|
| 2
|
7
|
Tue Apr 7
|
LS
|
SG
|
Day 7: cDNA synthesis and microarray
|
* M2D7 Lecture
* M2D7 Pre-Lab
|
| 2
|
8
|
Thur Apr 9
|
LS
|
SG
|
Day 8: Microarray data analysis
|
* M2D8 Lecture
* M2D8 Pre-Lab, ANS
* M2D8 Pre-Lab, SJG
|
|
|
| 3
|
1
|
Tues Apr 14
|
ANS
|
LJ
|
Day 1: Start-up biomaterials engineering
|
Module 2 Draft Due *see update*
* M3D1 Lecture
* M3D1 Pre-Lab
|
| 3
|
2
|
Thur Apr 16
|
ANS
|
LJ
|
Day 2: Initiate cell culture
|
* M3D2 Lecture
|
|
|
|
Patriot's Day April 20-21
|
No lab Apr 21 or Apr 22
|
| 3
|
3
|
Thur Apr 23
|
ANS
|
LJ
|
Day 3: Testing cell viability
|
* M3D3 Lecture
|
| 3
|
4
|
Tue Apr 28
|
ANS
|
LJ
|
Day 4: Preparing cells for analysis
|
* M3D4 Lecture
* M3D4 Pre-Lab
|
| 3
|
5
|
Thur Apr 30
|
ANS
|
LJ
|
Day 5: Transcript-level analysis
|
Module 2 Final Report Due
* M3D5 Lecture
* M3D5 Pre-Lab
|
| 3
|
6
|
Tue May 5
|
ANS
|
LJ
|
Day 6: Protein-level analysis
|
*Atissa's slides on her user page
* M3D6 Pre-Lab
|
| 3
|
7
|
Thur May 7
|
ANS
|
LJ
|
Day 7: Wrap-up analysis
|
Module 3 Report Due
Chalkboard lecture on drug + gene delivery (won't be posted).
* M3D7 Pre-Lab
|
| 3
|
8
|
Tue May 12
|
ANS
|
LJ
|
Day 8: Student presentations
|
Chalkboard lecture: Module 3 analysis/results, scaffold fabrication, clinical examples (won't be posted).
|
|
|
|
Thur May 14
|
|
|
No lab session. Evaluations during lecture, then celebratory lunch, in 56-614 from 12:30-2 pm.
|
|
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