User contributions for Skim

22 August 2007

• 14:4114:41, 22 August 2007 diff hist +446‎ IGEM:MIT/2007/Notebook/2007-8-22 ‎ →‎Agenda

9 August 2007

• 14:3814:38, 9 August 2007 diff hist −1‎ IGEM:MIT/2007/Notebook/2007-8-9 ‎ →‎Accomplished Today current
• 14:3714:37, 9 August 2007 diff hist +7‎ IGEM:MIT/2007/Notebook/2007-8-9 ‎ →‎Miniprepped Standard Assembly LCs
• 14:3714:37, 9 August 2007 diff hist +4‎ IGEM:MIT/2007/Notebook/2007-8-9 ‎ →‎Miniprepped Standard Assembly LCs
• 14:3614:36, 9 August 2007 diff hist +908‎ N IGEM:MIT/2007/Notebook/2007-8-9 ‎ New page: ===Accomplished Today=== ==Miniprepped Standard Assembly LCs== *12 tubes, 4 each of CPX/B0014, I14032/B0034 and R0051/B0034, all in DH5alpha *NOTE: Elution with Tris EDTA, not water *Nanod...

7 August 2007

• 13:0913:09, 7 August 2007 diff hist +175‎ IGEM:MIT/2007/Notebook/2007-8-7 ‎No edit summary current

30 July 2007

• 12:5812:58, 30 July 2007 diff hist +8‎ IGEM:MIT/2007/Notebook/2007-7-30 ‎ →‎Live Cell Immunoassay (Eric)
• 11:3111:31, 30 July 2007 diff hist +31‎ IGEM:MIT/2007/Notebook/2007-7-30 ‎ →‎Live Cell Immunoassay (Eric)
• 11:3011:30, 30 July 2007 diff hist +665‎ IGEM:MIT/2007/Notebook/2007-7-30 ‎No edit summary

24 July 2007

• 13:4413:44, 24 July 2007 diff hist +537‎ IGEM:MIT/2007/Notebook/2007-7-24 ‎ →‎AGENDA
• 13:3713:37, 24 July 2007 diff hist +685‎ IGEM:MIT/2007/Notebook/2007-7-24 ‎ →‎AGENDA
• 06:3906:39, 24 July 2007 diff hist +548‎ N IGEM:MIT/2007/Notebook/2007-7-24 ‎ New page: ==AGENDA== *Plate the Mer/I13500 3A transformation after overnight ligation (and grow) *Check if sequence results are available from Monday (length was correct) *Second polystyrene assay (...

23 July 2007

• 12:3512:35, 23 July 2007 diff hist +19‎ IGEM:MIT/2007/Notebook/2007-7-23 ‎ →‎3A Ligation of Mer promoter+Mer regulatory protein (pTO40) and RBS+GFP (I13500) into pSB1AT3 (SK, JH, BH)
• 12:3412:34, 23 July 2007 diff hist 0‎ IGEM:MIT/2007/Notebook/2007-7-23 ‎ →‎Agenda
• 12:3312:33, 23 July 2007 diff hist −1‎ IGEM:MIT/2007/Notebook/2007-7-23 ‎ →‎Agenda
• 12:3212:32, 23 July 2007 diff hist +37‎ IGEM:MIT/2007/Notebook/2007-7-23 ‎ →‎3A Ligation of Mer promoter+Mer regulatory protein (pTO40) and RBS+GFP (I13500) into pSB1AT3
• 12:3012:30, 23 July 2007 diff hist +319‎ IGEM:MIT/2007/Notebook/2007-7-23 ‎ →‎3A Ligation of Mer promoter+Mer regulatory protein (pTO40) and RBS+GFP (I13500) into pSB1AT3
• 12:2612:26, 23 July 2007 diff hist +800‎ IGEM:MIT/2007/Notebook/2007-7-23 ‎ →‎Digestion of I13500

20 July 2007

• 12:2912:29, 20 July 2007 diff hist +45‎ IGEM:MIT/2007/Notebook/2007-7-20 ‎ →‎Agenda current
• 12:2812:28, 20 July 2007 diff hist +13‎ IGEM:MIT/2007/Notebook/2007-7-20 ‎ →‎Agenda
• 12:2812:28, 20 July 2007 diff hist +227‎ IGEM:MIT/2007/Notebook/2007-7-20 ‎ →‎Agenda

19 July 2007

• 08:4508:45, 19 July 2007 diff hist +398‎ IGEM:MIT/2007/Notebook/2007-7-19 ‎ →‎Agenda for Thursday, 7/19

18 July 2007

• 12:3212:32, 18 July 2007 diff hist +43‎ IGEM:MIT/2007/Notebook/2007-7-19 ‎ →‎Agenda for Thursday, 7/19
• 12:3012:30, 18 July 2007 diff hist +6‎ IGEM:MIT/2007/Notebook/2007-7-19 ‎ →‎Agenda for Thursday, 7/19
• 12:3012:30, 18 July 2007 diff hist +61‎ N IGEM:MIT/2007/Notebook/2007-7-19 ‎ New page: ===Agenda for Thursday, 7/19=== *Plating (K+/A+)? *AK/AL list
• 09:4809:48, 18 July 2007 diff hist 0‎ IGEM:MIT/2007/Notebook/2007-7-18 ‎ →‎Agenda
• 09:4709:47, 18 July 2007 diff hist +385‎ IGEM:MIT/2007/Notebook/2007-7-18 ‎ →‎Agenda

17 July 2007

• 09:0809:08, 17 July 2007 diff hist +419‎ IGEM:MIT/2007/Notebook/2007-7-17 ‎ →‎Digestion of (F2620 and B0034) for 3A

14 July 2007

• 23:4423:44, 14 July 2007 diff hist +1‎ IGEM:MIT/2007/Notebook/2007-7-14 ‎ →‎Weekend Update
• 23:4323:43, 14 July 2007 diff hist +28‎ IGEM:MIT/2007/Notebook/2007-7-14 ‎ →‎Late Friday (7/13) Afternoon Update
• 23:4323:43, 14 July 2007 diff hist +1,908‎ N IGEM:MIT/2007/Notebook/2007-7-14 ‎ New page: ===Late Friday (7/13) Afternoon Update=== *BH/SK put dry pT040 (?) plates in warm room (for AK and AL) *Tom stopped by -- we have a BUNCH of chemically competent cells in the -80 degree fr...

11 July 2007

• 11:0411:04, 11 July 2007 diff hist +443‎ IGEM:MIT/2007/Room Reservations ‎No edit summary

9 July 2007

• 10:4810:48, 9 July 2007 diff hist +1‎ IGEM:MIT/2007/Notebook/2007-7-3 ‎ →‎Incubated plates of DH5a current
• 08:0408:04, 9 July 2007 diff hist +4‎ IGEM:MIT/2007/Notebook/2007-7-3 ‎ →‎Make LB+KAN agar plates

7 July 2007

• 14:4314:43, 7 July 2007 diff hist +458‎ IGEM:MIT/2007/Notebook/2007-7-7 ‎ →‎Checked Plates 5 PM current

2 July 2007

• 14:3514:35, 2 July 2007 diff hist +37‎ IGEM:MIT/2007/Notebook/2007-7-3 ‎ →‎Agenda for July 3
• 14:3514:35, 2 July 2007 diff hist +41‎ IGEM:MIT/2007/Notebook/2007-7-3 ‎ →‎Agenda for July 3
• 14:3414:34, 2 July 2007 diff hist +140‎ N IGEM:MIT/2007/Notebook/2007-7-3 ‎ New page: ===Agenda for July 3=== *Call Geneart *Results of Alex/Diti PCR? *For metals: RBS and promoter? (clarify!) *Barry's promoter -- obtainment?

1 July 2007

• 20:1920:19, 1 July 2007 diff hist +27‎ IGEM:MIT/2007/Notebook/2007-7-2 ‎ →‎Agenda: Monday, 7/2

29 June 2007

• 13:2513:25, 29 June 2007 diff hist +39‎ N IGEM:MIT/2007/Notebook/2007-7-2 ‎ New page: ===Agenda: Monday, 7/2=== *Order stuff!

28 June 2007

• 22:3022:30, 28 June 2007 diff hist +507‎ N IGEM:MIT/2007/Notebook/2007-6-29 ‎ New page: ===Agenda for Friday, June 29=== *Morning **pBAD pickup **Is OmpA crucial to bacterial cell survival? **Finalize oligos (restriction sites) **Research "red team" stuff -- ion exchange **Br...

25 June 2007

• 12:1712:17, 25 June 2007 diff hist +412‎ N Talk:IGEM:MIT/2007/Notebook/2007-6-25 ‎ New page: ===CHECKLIST for Monday, 6/25=== *Reorganize wiki *CPX/OmpC inserts **Polystyrene 17 **Polystyrene 18 (Belcher?) **Polypyrrole *Check sequences for CPX *Call Brian/grads/Eric check inserts... current
• 12:0512:05, 25 June 2007 diff hist +686‎ N IGEM:MIT/2007/Notebook/2007-6-25 ‎ New page: ===USING SEQUENCHER (Demo)=== *Copy desired gene sequence (to check) *Sequence tab **Create New Sequence **Give name **Paste sequence *View tab **Translation – different reading frame...

20 June 2007

• 07:5007:50, 20 June 2007 diff hist +742‎ IGEM:MIT/2007/Ideas3 ‎ →‎Mussel Protein

17 June 2007

• 21:4721:47, 17 June 2007 diff hist +1‎ IGEM:MIT/2007/Notebook/2007-6-17 ‎ →‎Notes from Meeting at 8 pm current
• 21:4621:46, 17 June 2007 diff hist +2,971‎ IGEM:MIT/2007/Notebook/2007-6-17 ‎No edit summary

11 June 2007

• 20:1120:11, 11 June 2007 diff hist 0‎ IGEM:MIT/2007/Notebook/2007-6-11 ‎ →‎Making Plates
• 20:1120:11, 11 June 2007 diff hist −2‎ IGEM:MIT/2007/Notebook/2007-6-11 ‎ →‎Transforming DNA plasmids into bacterial colonies on plates
• 20:0920:09, 11 June 2007 diff hist −2‎ IGEM:MIT/2007/Notebook/2007-6-11 ‎ →‎General Lab Do s and Don’t s
• 20:0820:08, 11 June 2007 diff hist −10‎ IGEM:MIT/2007/Notebook/2007-6-11 ‎ →‎General Lab Do s and Don’t s