Escherichia coli
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Contents |
[edit] General pages
- Bacterial artificial chromosomes
- E. coli genotypes
- E. coli restriction-modification system
- Endy:High-efficiency electroporation
- Escherichia coli/Codon usage
- Escherichia coli/Knockouts
- Escherichia coli/Methylation
- Escherichia coli/Nomenclature & Abbreviations
- Escherichia coli/Vectors
- F plasmids
- Klapperich Lab:Streaking bacteria on Agar plate
- Recombineering
- Standard E. coli Strain for BioBricks
- Titratable control of pBAD and lac promoters in individual E. coli cells
- Transposable elements
[edit] Protocols
- Bacterial cell culture
- Bacterial transformation
- Beta-Galactosidase Assay (A better Miller)
- Cell counting/plating
- Colony PCR
- Electrocompetent cells
- Electroporation
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- Making a long term stock of bacteria
- Maxiprep of plasmid DNA from E. coli
- Miniprep
- Miniprep/GET buffer
- Miniprep/Kit-free high-throughput protocol
- Miniprep/Qiagen kit
- One step 'miniprep' method for the isolation of plasmid DNA
- P1vir phage transduction
- Preparing chemically competent cells
- Preparing chemically competent cells (Inoue)
- Recombineering/Lambda red-mediated gene replacement
- TOP10 chemically competent cells
- Transforming chemically competent cells
- Transforming chemically competent cells (Inoue)
- Belcher/Knight: Electrocompetent Cells
- Endy:Colony PCR
- Endy:E. coli Western Blot
- Endy:STN Chemostat Protocol
- Knight:Beta-galactosidase assay
- Knight:Beta-galactosidase assay/96 well format
- Knight:Beta-galactosidase assay/Fluorescence
- Knight:Colony PCR
- Knight:DNA Spots
- Knight:Electroporation
- Knight:Miniprep low copy plasmids
- Knight:Preparing electrocompetent cells
- Lory:Quick transformant screen
- Sauer:Lysing E. coli with Lysozymes
- Seth:Quick Transformant Screen
- Streptomyces:Protocols/Mini-Maxi Prep
- Streptomyces:Protocols/PCR
- Streptomyces:Protocols/Transformation by Electroporation
[edit] Materials
- Endy:M9 media/supplemented
- Escherichia coli RNA polymerase
- Knight:M9 supplemented media
- M9 medium
- M9 medium/minimal
- M9 medium/supplemented
- M9 medium/with agar
- Streptomyces:Other Bits/Antibiotic and Resistance Marker Information
- TSS
[edit] Notes
- In an Escherichia coli cell, 1 molecule per cell is approximately equal to 1nM.
[edit] Resources
- EcoliWiki.org
- A community annotation site for E. coli K-12. Includes its phages, plasmids, genes, strains and databases.
- Escherichia coli statistics by Institute for Biomolecular Design
- Several tables describing statistical data on E. coli compiled from several sources.
- Physiology of the Bacterial Cell by Neidhardt, Ingraham and Schaechter
- Reference text on bacterial cell physiology.
- EcoSal by ASM Press
- An incomplete web version of the publication Escherichia coli and Salmonella: Cellular and Molecular Biology. [Subscription required]
- EcoGene
- Database of all E. coli genes and sequences
- Ecocyc
- Comprehensive biochemical pathway and gene function site for E. coli
[edit] References
- Cohen SN, Chang AC, and Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A 1972 Aug; 69(8) 2110-4. pmid:4559594.
This appears to be one of the earliest papers on transformation of Escherichia coli. If you find an earlier paper, please include it here.
