Escherichia coli RNA polymerase

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Epicentre is the only commercial source for Escherichia coli RNA polymerase.

Contents 1 Materials 2 Reaction conditions 2.1 For core enzyme 2.2 For sigma saturated holoenzyme 2.2.1 Functional test 2.2.2 Assay test

[edit] Materials

• E. coli RNA Polymerase Core Enzyme and Sigma-Saturated Holoenzyme from Epicentre

[edit] Reaction conditions

[edit] For core enzyme

from Epicentre

[edit] For sigma saturated holoenzyme

from Epicentre

[edit] Functional test

50 μL reaction

• 1X E. coli RNA polymerase transcription buffer
  • 0.04 M Tris-HCl (pH 7.5)
  • 0.15 M KCl
  • 10 mM MgCl₂
  • 0.01% Triton® X-100
• 2mM DTT
• 0.25mM NTPs
• 1μg T7 D111 DNA as template

[edit] Assay test

50 μL reaction

• 1X E. coli RNA polymerase transcription buffer
  • 0.04 M Tris-HCl (pH 7.5)
  • 0.15 M KCl
  • 10 mM MgCl₂
  • 0.01% Triton® X-100
• 10 mM DTT
• 0.5 mM NTPs
• 1μg T7 D111 DNA as template