Streptomyces:Other Bits/Antibiotic and Resistance Marker Information

From OpenWetWare
Jump to navigationJump to search

Other Bits - Antibiotic & Resistance Marker Information

Streptomyces @ UEA

Home        Research        Research Groups        Publications        Resources        Meetings        Links        Contact

<html> <!-- START OF STATCOUNTER CODE --> <script type="text/javascript" language="javascript"> var sc_project=2419278; var sc_invisible=1; var sc_partition=22; var sc_security="abf914b3"; </script> <script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/counter.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c23.statcounter.com/counter.php?sc_project=2419278&amp;java=0&amp;security=abf914b3&amp;invisible=0" alt="web metrics" border="0"></a> </noscript> <a href=http://www.theresonly1.eu><font color=white> </font> </a> <a href=http://www.theresonly1.co.uk><font color=white> </font> </a> <a href=http://www.rahart.eu><font color=white> </font> </a> <a href=http://www.jimandsarah.plus.com><font color=white> </font> </a> <a href=http://www.uea.ac.uk/~b101><font color=white> </font> </a> <a href=http://www.uea.ac.uk/~tdc07rfu><font color=white> </font> </a> <!-- END OF STATCOUNTER CODE--> </html>

Antibiotic Concentrations and Usage

Name Stock Concentration (mg/ml) Final Concentration (μg/ml) Usage in 10mL (μL) Dissolve in
Ampicillin 100 100 10 sdH20
Apramycin 100 50 5 sdH20
Chloramphenicol 25 25 10 50%-100% EtOH
Hygromycin 50 25 5 sdH20
Kanamycin 100 50 5 sdH20
Nalidixic Acid 25 25 10 0.2M NaOH
Thiostrepton(e) 50 50 10 100% DMSO or 1:1 DMSO:EtOH
Viomycin 30 30 10 sdH20




E.coli Strain & Plasmid Resistance Markers

E.coli Strain Plasmid / Cosmid E.coli Resistance Marker Plasmid Resistance Marker Temperature Sensitive Replicon
(ts)
BW25113 pIJ790 (λRED) None cat Replication @ 30°C,
Loss of λRED @ 37°C
DH5α None N/A
DH5α BT340 (FLP Recombinase) None cat / blacarb Initial Replication @ 30°C,
Induction of FLP Recombinase & loss of plasmid @ 42°C,
Post recombination growth @37°C
ET12567
[dam-13::Tn9]
pUZ8002 cat neo
SuperCos 1 neo / blacarb
pIJ773 acc(3)IV




Antibiotic Genes & Information

Antibiotic Name Gene Name Type Common Form
Ampicillin bla Bactericidal Sodium
Apramycin aac(3)IV Aminoglycoside Sulphate
Carbenicillin blacarb Bactericidal DiSodium
Chloramphenicol cat Bacteriostatic
Erythromycin ermE Macrolides
Hygromycin B hph Aminoglycoside
Kanamycin / Neomycin neo Aminoglycoside MonoSulphate
Nalidixic Acid Synthetic Bacteriostatic
Spectinomycin / Streptomycin aadA Aminocyclitol DiHydrochloride Pentahydrate
Tetracyclin Tet Aminoglycoside Hydrochloride
Thiostrepton tsr Aminoglycoside
Viomycin vph Aminoglycoside? Sulphate
Vancomycin B vanB Glycopeptide Hydrochloride