Escherichia coli/Knockouts

From OpenWetWare

The Keio collection is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the Functional Genomic Analysis of E. coli in Japan project [1].

You can request strains from them.

Request form - note that they are quoting a shipping delay of 2 months due to so many requests.

Alternatively, you can obtain the relevant plasmids for doing the knockouts yourself from the CGSC (pKD13 and pKD46 are needed most likely)[2].

References

1. Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, and Mori H. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2006; 2 2006.0008. doi:10.1038/msb4100050 pmid:16738554. PubMed HubMed [Baba-MSB-2006]
2. Datsenko KA and Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 2000 Jun 6; 97(12) 6640-5. doi:10.1073/pnas.120163297 pmid:10829079. PubMed HubMed [Datsenko-PNAS-2000]

Source

• Email correspondence between John Cumbers and Wilson Wong.