Xylene cyanol
From OpenWetWare
Jump to navigationJump to search
Xylene cyanol (XC) is most commonly used as an agarose gel electrophoresis size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than bromophenol blue (670g/mol) because of its charge (1 net negative charge).
- 10'000 bp at 0.7%
- 4000 bp at 1.5%
- 750 bp at 2.0%
- 200 bp at 3.0%
order information
- CAS 2650-17-1
- e.g. Sigma Xylene Cyanol FF X4126 10g at around €50
see also
- agarose gel loading buffer
- agarose gel electrophoresis
- Cresol Red - faster than XC
- Bromophenol blue - faster than XC
