Cresol Red

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chemical structure of cresol red

Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.

Agarose molecular weight marker

advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue

  • molecular weight 404 g/mol
  • in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
  • in 3% agarose at >100bp (xylene cyanol 300bp)

pH indicator

  • amber - acidic pH
  • yellow - neutral
  • red - alkaline

External links