Xist (ncRNA)

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BMCB625 Advanced Topics in Molecular Biology


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(Homework) Questions



  1. How do Chaumeil, et al., track a "single species" of mRNA transcripts?
  2. How to they demonstrate the absence of transcription? What is a major drawback to this method?

--Chris 22:22, 23 April 2007 (EDT)


Q1. How do you think the Xist transcript is able to exclude RNA Pol II and other transcription components from a "defined" region? How would you go about testing your hypothesis?

Q2. In relation to Q1 - what do you think the Xist domain is comprised of?