User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/10/05

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Ocean Optics Round 4

pH 7

Volumes and concentrations: Screenshot 2016-10-05 at 12.50.46 PM.png

We accidentally set the temperature to room temperature instead to 80 degrees. No increase in absorption occurred even after an hour and a half, so almost no nano particles were formed. Have to re-run pH 6 Ocean Optics.


Data Analysis

Oceanoptics161006pH7BSA.PNG

The above chart shows the UV-Vis maximum absorbance over time done on the Ocean Optics instrument of pH 7 Au NP with BSA=3.125 uM. The chart shows that pH 7 formed many fewer NPs than other pH solutions have in the past.


Oceanoptics161006pH7BSAA530.PNG

The above chart shows the UV-Vis A(535.15) over time done on the Ocean Optics instrument of pH 7 Au NP with BSA=3.125 uM. The chart shows that pH 7 formed many fewer NPs than other pH solutions have in the past, but there is still a trend showing an increase over time.


Oceanoptics161006pH7BSAWAVELENGTH.PNG

The chart above shows an increase in the maximum wavelength around 7000 sec. The readings making up this chart are much "shakier" than previous runs.

Lysozyme presentation

  • absorbance jump at 30 min

pH 5: shifted to left. dip in absorbance. pH 6 absorbance lower. start doing: change from time 0. drop them together. absorption of single wavelength vs time. at 30 minutes ~ 560 nm you see jump. absorbing at longer wavelengths . stability of protein at low pH.

integrated intensity: increases , decreases , increases. emission shifting to right with time. monitoring a specific wavelength. compare to integrated intensity which has a shifting maximum. overlap kinetics with absorbance. have to compare max intensity for absorbance : allowing fluorescence shift but not UV shift. max absorbance. many chemical processes. fluorescence dip: time at which making nanoparticles (35 min) no absorption features stand out before, but emission stands out . protein - gold interaction . at 5 min: broader fluorescence. luminescent gold complexes. unfolded within first 5 minutes. add graph for just protein. start thinking of deconvolute fluorescence in diff states. one wavelength is increasing . large peak at 380 -- overlap. component analysis. software ? areas under curve vs time. fit to 4 - 5 Gaussians.