User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/26

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Fluorescence Purpose Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 8 Procedure Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total Au 2.93mM (0.25mM)(3.0mL)=(XmL)(2.93mM) XmL= .256mL= 256uL Au Myoglobin 133uM (12.5uM)(3.0mL)=(XmL)(133uM) X=0.282mL= 282uL Myoglobin 3uL 1mM NaOH Water Volume of water= 3000uL- 256uL-282uL-3uL= 2459uL Absorbance measurements were taken and saved every 3 minutes for 3 hours The instrument was set at 80 degrees Celsius The scan range parameters were start at 310nm and ending at 540nm The emission and exit slit were set at 10nm Data Scans AuNP pH 8 Wavelength of Myoglobin AuNp's at pH 8 Maximum Intensity of Myoglobin AuNp's at pH 8 Integrated Intensity of Myoglobins AuNp's at pH 8 Solution for UV-Vis Preparation Stock Concentrations: Myoglobin 70uM for 0.75mM Fructose solutions, 67.76uM for 1.25mM Fructose solution Silver 25.8mM Fructose 33.0mM 0.75mM Fructose pH μL of 1 mM HNO3stock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AgNO33stock μL of Fructosestock μL of Myoglobinstock μL of Water 4 500 0 0 48 113 886 3451 5 50 0 0 48 113 886 3901 6 5 0 0 48 113 886 3946 7 0 0 0 48 113 886 3951 8 0 5 0 48 113 886 3946 9 0 50 0 48 113 886 3901 10 0 500 0 48 113 886 3451 11 0 0 5 48 113 886 3946 12 0 0 50 48 113 886 3901 1.25 mM Fructose pH μL of 1 mM HNO3stock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AgNO33stock μL of Fructosestock μL of Myoglobinstock μL of Water 4 500 0 0 48 189 922 3339 5 50 0 0 48 189 922 3789 6 5 0 0 48 189 922 3834 7 0 0 0 48 189 922 3839 8 0 5 0 48 189 922 3834 9 0 50 0 48 189 922 3787 10 0 500 0 48 189 922 3339 11 0 0 5 48 189 922 3834 12 0 0 50 48 189 922 3789