User:Sara K. Mattson/Notebook/Chemistry 671/2016/11/01

From OpenWetWare
Jump to navigationJump to search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

UV-VIS

Purpose

To observe the change in absorbance of AgNO3 NP's at pH 4-12 for fructose at concentrations 0.75mM and 1.25mM

Procedure

  1. Solutions were prepared on 10/26/2016
    1. The solutions had been heated in an 80C oven for four hours and then sat at room temperature. UV-Vis spectra were taken of each sample from 200-800nm.
    2. The test tubes were kept completely wrapped in tin foil the entire time, because AgNO3 is light sensitive.

Solutions

  1. All solutions at pH 4-10 for both concentrations of fructose, 0.75mM and 1.25mM were a pale yellow color with some cloudiness.
  2. Solutions at pH 11 and 12 for both concentrations were a bright orange color with some amount of cloudiness
  3. Below are the samples at pH 11 and 12 from all six concentrations of fructose (0mM, 0.0625mM, 0.125mM, 0.25mM, 0.75mM and 1.25mM)

PH11 AgNP myoglobin.jpg

PH12 AgNP myoglobin.jpg

Data

  1. Graphs for 0.75mM Fructose

Absorbance vs Wavelength

Absorbance vs wavelength uv-vis 0.75 fructose.png

Absorbance at 400nm

Absorbance at 400nm 0.75mM fructose.png

  1. Graphs for 1.25mM Fructose

Absorbance vs Wavelength

1.25mM fructose absorbance vs wavelength.png

Absorbance at 400nm

1.25mM fructose absorbance at 400nm.png