User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/04/21

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Spermatogenesis

  • Vizualized slides.
    • ubi-RFP localization was as expected. There were no areas without expression; however, there were a few cells near the hub with high expression levels. Background noise, however, was very high.
    • rfp-h2au Cyclin-B Stain was inconclusive. Too much background noise. Will dissect more flies and stain again with varying concentrations this week.
      • Potentially 1:25, 1:50, 1:100. Dissect ~30 flies. 10 per concentration.